CAREER: Multi-scale Imaging of Neural Interconnects using MRI-compatible Parallel Photonic Needle Interface

职业：使用 MRI 兼容的并行光子针接口对神经互连进行多尺度成像

• 批准号: 1254743
• 负责人: Yu Chen
• 金额: $ 40万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2019-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1254743&HistoricalAwards=false
• 关键词: CAREER; Multi; scale; Imaging; Neural

Abstract#1254743ChenThe long-term research goal is to establish a new multi-modal imaging platform that combines optical imaging and MRI for multi-scale parallel imaging of neural activity. As a step towards this goal, the proposed research objectives in this CAREER proposal are: 1) Investigate depth-resolved neural physiological changes using multi-contrast OCT; 2) Implement MRI-compatible parallel photonic needle interface (PPNI) for simultaneous MRI/OCT; 3) Establish multi-scale MRI/PPNI-OCT imaging in liveanimals. They will apply this novel imaging technique to the mouse trigeminal system, which is associated with patterned array of whisker-related neural modules in various brain structures, and provides an excellent model system to investiate cellular and molecular mechanisms of neural pattern formation and plasticity. They will image populations of whisker-related neurons in the cortex and subcortical trigeminal centers using PPNI-OCT/MRI, which will allow to simultaneously investigate whisker-evoked activity patterns in the brainstem, thalamus, and cortex.

长期研究目标是建立一个新的多模式成像平台，将光学成像和磁共振成像相结合，用于神经活动的多尺度并行成像。作为迈向这一目标的一步，这份职业提案中提出的研究目标是：1)使用多对比OCT研究深度分辨的神经生理变化；2)实现与MRI兼容的并行光子针接口(PPNI)，用于同时进行MRI/OCT；3)建立活体动物的多尺度MRI/PPNI-OCT成像。他们将把这种新的成像技术应用于小鼠三叉神经系统，该系统与不同大脑结构中与胡须相关的神经模块的图案阵列相关，并提供了一个很好的模型系统来研究神经图案形成和可塑性的细胞和分子机制。他们将使用PPNI-OCT/MRI成像皮质和皮质下三叉神经中心中与胡须相关的神经元群体，这将允许同时研究脑干、丘脑和皮质中胡须诱发的活动模式。