HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE -STIMULATING HORMONE SUBUNITE GENE
HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE -STIMULATING HORMONE SUBUNITE GENE
批准号:
12557087
负责人:
KATO Yukio
金额:
$6.14万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2000
资助国家:
日本
项目状态:
已结题
起止时间:
2000 至 2003
中文摘要
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英文摘要
(1)FOUR TRANSCRIPTION FACTORS THAT BIND TO THE UPSTREAM REGION OF PORCINE FOLLICLE STIMULATING HORMONE SUBUNIT GENE HAVE BEEN CLONED USING YEAST ONE-HYBRID SYSTEM.(2)THE NUCEOTIDE SEQUENCES OF CLONES HAVE HOMEOTIC REGION, WHICH RECOGNIZES SPECIFIC DNA SEQUENCES.(3)PRODUCTION OF RECOMBINANT TRANSCRIPTION FACTOR HAVE BEEN SUCCEEDED AND THE GEL MOBILITY SHIFT ASSAY DEMONSTRATED SPECIFIC BINDING FOR FSHβ GENE(4)EXPRESSION. VECTORS CONTAINING CLONED TRANSCRIPTION FACTORS WERE TRANSFECTED IN THE CHO CELLS TOGETHER WITH A REPORTER VECTOR FUSED WITH THE 5'-UPSTREAM REGION OF THE FSHI3 GENE AND SOME STIMULATION OF THE GENE EXPRESSIO WAS OBSERVED.
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Moriyama, R., Tsukamura, H., Kinoshita, M., Okazaki, H., Kato, Y., Maeda, K.-I.: "In vitro Increase in Intracellular Calcium Concentrations Induced by Low or High Extracellular Glucose Levels in Ependymocytes and Serotonergic Neurons of the Rat Lower Brai
Moriyama, R.、Tsukamura, H.、Kinoshita, M.、Okazaki, H.、Kato, Y.、Maeda, K.-I.:“细胞外葡萄糖水平低或高引起的体外细胞内钙浓度增加”
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Iwasawa A: "Specific anti-peptide antibody to β subunit of chicken thyrotropin : production and characterization"Endocr J. 48. 197-204 (2002)
Iwasawa A:“针对鸡促甲状腺素β亚基的特异性抗肽抗体:生产和表征”Endocr J. 48. 197-204 (2002)
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Tomizawa K: "Production and Characterization of an Antiserum against Recombinant Porcine Follicle Stimulating Hormone"J Reprod Dev 2002;48:131-136. 48. 131-136 (2002)
Tomizawa K:“针对重组猪卵泡刺激激素的抗血清的生产和表征”J Reprod Dev 2002;48:131-136。
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Kato Y: "Presence of an 85 kDa pituitary protein (Pit-G) in porcine GH-producing cells"J Reprod Dev 2002 ; 48 : 87-95. 48. 87-95 (2002)
Kato Y:“猪 GH 生成细胞中存在 85 kDa 垂体蛋白 (Pit-G)”J Reprod Dev 2002;
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Nishida, N.: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation"Journal of Molecular Endocrinology. 26. 259-265 (2001)
Nishida, N.:“FSH 刺激后培养的猪颗粒细胞中内皮一氧化氮合酶基因的表达”分子内分泌学杂志。
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依托单位:
HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE
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PRODUCTION OF RECOMBINANT GONADOTROPINS (FSH AND LH) AND APPLICATION FOR CONTROL IN REPRODUCTION
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