HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE
HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE
批准号:
12470217
负责人:
KATO Yukio
金额:
$9.09万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2000
资助国家:
日本
项目状态:
已结题
起止时间:
2000 至 2002
中文摘要
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英文摘要
(1) FOUR TRANSCRIPTION FACTORS THAT BIND TO THE UPSTREAM REGION OF PORCINE FOLLICLE STIMULATING HORMONE SUBUNIT GENE HAVE BEEN CLONED USING YEAST ONE-HYBRID SYSTEM.(2) THE NUCEOTIDE SEQUENCES OF CLONES HAVE HOMEOTIC REGION, WHICH RECOGNIZES SPECIFIC DNA SEQUENCES.(3) PRODUCTION OF RECOMBINANT TRANSCRIPTION FACTOR HAVE BEEN SUCCEEDED AND THE GEL MOBILITY SHIFT ASSAY DEMONSTRATED SPECIFIC BINDING FOR FSH β GENE(4) EXPRESSION VECTORS CONTAINING CLONED TRANSCRIPTION FACTORS WERE TRANSFECTED IN THE CHO CELLS TOGETHER WITH A REPORTER VECTOR FUSED WITH THE 5'-UPSTREAM REGION OF THE FSHβ GENE AND SOME STIMULATION OF THE GENE EXPRESSIO WAS OBSERVED.
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Iwasawa A: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)"J Reprod Dev 2002;48:489-496. 48. 489-496 (2002)
Iwasawa A:“在母鸡诱导换羽过程中促甲状腺素信使 RNA 和循环激素水平的变化”J Reprod Dev 2002;48:489-496。
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Nishida, N.: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation"Jounal of Molecular Endocrinology. 26. 259-265 (2001)
Nishida, N.:“FSH 刺激后培养的猪颗粒细胞中内皮一氧化氮合酶基因的表达”分子内分泌学杂志。
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Hattori, M-A.: "Expression of endothelial nitric oxide synthetase in the porcine oocyte and its possible function"Molecular and Cellular Biology. 219. 121-126 (2001)
Hattori,M-A.:“猪卵母细胞中内皮一氧化氮合成酶的表达及其可能的功能”分子和细胞生物学。
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Nishida,N.: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels"Experimental and Clinical Endocrinology and Diabetes. 108. 424-429 (2000)
Nishida,N.:“一氧化氮在转录和转录后水平猪 LH 受体表达中的关键作用”实验和临床内分泌学和糖尿病。
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Nishida,N.: "Transcription facgtor genes, c-fos and c-jun, in developing porcine granulosa cells in vitro"Journa of Reproduction and Development. 46. 167-175 (2000)
Nishida,N.:“转录因子基因 c-fos 和 c-jun 在体外培养猪颗粒细胞中的作用”《生殖与发育杂志》。
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HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE -STIMULATING HORMONE SUBUNITE GENE
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