PRODUCTION OF RECOMBINANT GONADOTROPINS (FSH AND LH) AND APPLICATION FOR CONTROL IN REPRODUCTION
PRODUCTION OF RECOMBINANT GONADOTROPINS (FSH AND LH) AND APPLICATION FOR CONTROL IN REPRODUCTION
批准号:
09556065
负责人:
KATO Yukio
金额:
$7.81万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1999
中文摘要
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英文摘要
(1) RECOMBINANT PORCINE BEEN SUCCESSIVELY PRODUCED AND THE BIOCHEMICAL CHARACTERISTICS WERE EXAMINED. THE BIOLOGICAL ACTIVITIES WERE OBSERVED.(2) EXPRESSION VECTOR FOR PORCINE LH WAS CONSTRUCTED AND EXAMINED LH PRODUCTION. THE RESULT SHOWED THE LOW LEVEL OF EXPRESSION AS FSH.(3) ANTIBODY FOR RECOMBINANT PORCINE FSH WAS PRODUCED. THE ANTISERUM REACTED WITH ONLY PORCINE FSH IN COMPETITIVE IMMUNOASSAY SYSTEM, INDICATING THAT THE ANTISERUM IS SPECIES-SPECIFIC. IN THE NON-COMPETITIVE USAGE SUCH AS IMMUNOHISTOCHEMISTRY, THE ANTI-RECOMBINANT PORCINE FSH ANTISERUM GAVE POSITIVELLY IMMUNOREACTIVE PITUITARY CELLS FOR SEVERAL MAMMALS.(4) USAGE OF RECOMBINANT PORCINE FSH FOR IN VITRO FERTILIZATION WAS EXAMINED. RECOMBINANT PORCINE FSH INDUCED THE GVBD OF PORCINE OOCYTES BUT NOT THAT OF BOVINE OOCYTES.
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加藤 幸雄: "組換え体性腺刺激ホルモンの生産と応用" 獣医畜産新報. 49. 39-43 (1988)
加藤幸雄:“重组促性腺激素的生产和应用”兽医畜牧报49。39-43(1988)。
DOI:
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作者:
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通讯作者:
Inaba T.: "Baculovirus-insect cell production of bioactive porcine FSH"Theriogenology. 47. 491-499 (1997)
Inaba T.:“具有生物活性的猪 FSH 的杆状病毒昆虫细胞生产”Theriogenology。
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通讯作者:
Kato, Y., Sato, I., Ihara, T., Tomizawa, K., Mori, J., Geshi, M., Nagai, T., Okuda, K., Kato, T. and Ueda, S.: "Expression of biologically active porcine follicle stimulating hormone in insect cells bearing a baculovirus vector and its purification."J. Mo
加藤 Y.、佐藤 I.、伊原 T.、富泽 K.、森 J.、Geshi, M.、永井 T.、奥田 K.、加藤 T. 和上田 S.:
DOI:
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作者:
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通讯作者:
Inaba,T.: "Baculovirus-insect cell production of bioactive porcine FSH" Theriogenology. 47. 491-499 (1997)
Inaba,T.:“具有生物活性的猪 FSH 的杆状病毒昆虫细胞生产”动物发生学。
DOI:
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作者:
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通讯作者:
加藤幸雄: "「ホルモンの分子生物学:第3巻 生殖内分泌」-ゴナドトロビン" 学会出版センター(印刷中),
加藤幸雄:《激素分子生物学:第三卷生殖内分泌》-促性腺激素”学会出版中心(正在印刷中),
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Roles of transporter adaptors as regulatory mechanism for drug absorption and disposition
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Molecular basis for quantitative prediction of drug-drug interaction at excretion process
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HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE
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Role of PTH and PTH-related peptide in skeletal development
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