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Focal depth extension and stereo viewing apparatus for high resolution microscope based on active image processing method

基于主动图像处理方法的高分辨率显微镜焦深扩展及立体观察装置

基本信息

批准号：
62850013
负责人：
IKUTA Takashi
金额：
$ 3.71万
依托单位：
Osaka Electro-Communication University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

A new technique of the focal depth extension for the high resolution optical microscope, with the aid of the active image processing method, has been proposed in the present study ( 1987 - 1988, Grant-in-Aid for Developmental Scientific Research 2 ).This focal depth extension technique is essentially based on Hausler's method ( 1972 )where moving defocus averaging and high pass filtering are applied to the observed microscopic image. The present technique, however, uses real time active deconvolution technique ( proposed by one of the authors ( 1985 ) as the active image processing method ) instead of the conventional high pass filter used in the Hausler's method. Main aims of this study are to construct a prototype focal depth extension system and to confirm its practicability.At the first stage of the study ( 1987 ), a fast 3D movable sample stage using piezo driving elements has been assembled and attached to the conventional optical microscope ( Olympus BHS ). Next, the previously constructed active image processing system ( include a frame integrator with bipolar weighting function and a personal computer ) has been connected to this sample stage via interface circuits. A stereo viewing subsystem using liquid crystal glasses has been also included in the active image processing system. By using this system, focal depth extension and stereo viewing experiments have been successfully achieved to transmission and reflection mode images of the microscope. Focal depth extension about 20 um defocus range has been obtained for the objective lens of N.A.= 0.7. Focal depth extension has been also applied to the phase microscope at the next stage ( 1988 ). Although the image formation of the phase microscope is some more complicated, successful focal depth extension and stereo viewing have been achieved with phase object by using a similar filtering function.

本文提出了一种利用主动图像处理方法实现高分辨光学显微镜焦深扩展的新技术（1987 - 1988年，发展科学研究补助金2）.这种焦深扩展技术基本上是以Hausler的方法（1972）为基础的其中将移动散焦平均和高通滤波应用于观察到的显微图像。然而，本技术使用真实的时间主动反卷积技术（由作者之一（1985）提出作为主动图像处理方法）来代替Hausler方法中使用的常规高通滤波器。本研究的主要目的是构建一个原型的焦深扩展系统，并确认其practicality.At研究的第一阶段（1987年），一个快速的三维可移动的样品台使用压电驱动元件已组装和连接到传统的光学显微镜（奥林巴斯BHS）。然后，将先前构建的主动图像处理系统（包括具有双极加权功能的帧积分器和个人计算机）通过接口电路连接到该样品台。使用液晶眼镜的立体观察子系统也被包括在主动图像处理系统中。利用该系统，成功地实现了透射和反射模式显微图像的焦深扩展和立体观察实验。对于N. A.=的物镜透镜，焦深扩展约20 μ m散焦范围0.7.焦深扩展也已应用于相位显微镜在下一阶段（1988年）。虽然相位显微镜的成像是一些更复杂的，成功的焦深扩展和立体观察已实现与相位对象通过使用类似的过滤功能。