Active multi-spectral aberration free phase microscope

本研究是基于过去对“科研资助”的研究结果。本研究的目的是利用主动式动态空锥照明及8字形（数值）带通滤波器，建构无像差的焦深扩展位相显微镜。根据这一目标，本研究的第一阶段（1997年），我们在光学显微镜上安装了一个主动式动态空心锥照明器。同时编写了照明控制、图像处理和网络应用程序，并对该系统进行了检验，成功地验证了其作为无像差焦深扩展位相显微镜的功能。利用几个样品对该系统的实用性进行了评价，除多光谱成像外，该系统的实用性良好。 ...更多信息 提出了一种基于主动动态空心锥照明和8字形带通滤波器的成像技术。目前的理论表明，除了焦深的延伸之外，主球差和高阶球差都被成功地校正。理论分析还表明，由于这种成像技术只观测到像的整体平移，所以主彗差的影响也可以忽略不计。在白色光源下对2种颜色染色的样品进行多光谱观察，显示颜色分离不足。这种不充分的颜色分离可能是由于非线性成像效应和针对不同波长的8字形带通滤波器的固有交叉。前者在高和中空间频率范围内降低了光谱分辨率，后者在低空间频率范围内降低了光谱分辨率。目前，对这种分色不足的分析和对策正在进行中。最后，将这种成像技术应用于透射电子显微镜是本研究的一部分。然而，由于机器时间的限制，使用透射电子显微镜的实验现在被安排在将来。少

The present study is based on results of past studies of 'Grant-in-aid for scientific research'. The aim of this study is the construction of aberration-free focal depth extended phase microscope using active dynamic hollow-cone illumination and figure (numerical) 8 shaped band-pass filter. And, the confirmation of the ability of the multi-spectral imaging is also important subject of this study.According to this aim, an optical microscope was reconstructed to attach an active dynamic hollow-cone illuminator at the first stage (1997) of this study. Programs for the illuminator control, the image processing and the network utility were also coded in this time, This system was then examined and successfblly confirmed its functions as the aberration-free focal depth extended phase microscope. Using several samples, the practicability of this system is evaluated as to be good except for the multi-spectral imaging.In the next stage (1998), the basic theory was established and reported for t … More he present imaging technique based on the active dynamic hollow-cone illumination and the figure 8 shaped band-pass filter. The present theory shows that principal and higher order spherical aberrations are, all, successfully corrected in addition to the extension of the focal depth. And the theory also shows that the influence of the principal coma aberration can be also neglected since only the entire image translation is observed using this new imaging technique.On the other hand, the experiment is not well achieved, as to the multi-spectral imaging. The multi-spectral observation of 2 color dyed sample under white light source shows insufficient color separation. This insufficient color separation is, probably, due to the non-linear imaging effect and the intrinsic crossing of the figure 8 shaped band-pass filter for different wave length. The former degrades the spectral resolution at high and median spatial frequency range, and the later degrades at the lower spatial frequency range. The analysis and the countermeasure of such insufficient color separation are, now, in progress.Finally, the application of this imaging technique to the transmission electron microscope is the part of present study. The experiment using transmission electron microscope is, however, now scheduled in future due to the restriction of the machine time. Less