Moire super resolution fluorescent microscopy based on active image processing

基于主动图像处理的莫尔超分辨率荧光显微镜

• 批准号: 63550051
• 负责人: IKUTA Takashi
• 金额: $ 1.22万
• 依托单位: Osaka Electro-Communication University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63550051/
• 关键词: Image Processing; Active Image Processing; Optical Microscope; Super Resolution System; 3D Imaging; Fluorescent Microscope; Moire; Real Time Image Processing; 3次元光学伝達関数

The present study has aimed to establish the theoretical background of the newly proposed moire super resolution fluorescent microscopy. Construction of the prototype system and the evaluation of the imaging characteristics have been also important subject of this study.At the first stage ( 1988 ), theoretical analysis has been developed to apply the moire super resolution technique to the fluorescent microscopy, which uses both 1-dimensional and 2-dimensional projection type masks. The analysis shows that the moire fluorescent microscopy has the characteristics of the in-plane super resolution and the true 3D resolution along the optical axis, which are similar to the characteristics of the confocal fluorescent microscope. Next, a prototype system has been constructed by attaching movable stages and moire/reconstruction masks to a conventional fluorescent microscope. Reconstruction experiment using 1-dimensional masks, under the mask double moving method based on the active image proc … More essing technique, has been attempted to biotic samples. From this experiment, the true 3D resolution along the optical axis has been well confirmed. On the other hand, it has been also found that the present moire fluorescent microscope is very sensitive and results in the contrast inversion with the misalignment between moire/reconstruction masks. The analysis and the proposal of the correction with this effect have been presented.The reconstruction experiment using 2-dimensional masks has been achieved and also confirmed the true 3D resolution at the next stage ( 1989 ) of the study. The evaluation of 3D OTF has been attempted by calculating the 3D spectra from observed trough defocus series. To increase brightness of the optical system, a phase mask has been introduced instead of the original amplitude mask.Although the confirmation of the super resolution rests in the future study in this time, the present moire fluorescent microscopy is expected to be useful means in the field of the biomedical application. Less

本研究的目的是建立新提出的莫尔超分辨荧光显微镜的理论背景。原型系统的构建和成像特性的评价也一直是本研究的重要课题。在第一阶段（1988年），理论分析已经发展到将莫尔超分辨技术应用于荧光显微镜，它使用1维和2维投影型掩模。分析表明，莫尔荧光显微镜具有与共焦荧光显微镜相似的面内超分辨和沿光轴沿着的真三维分辨特性。接下来，一个原型系统已被构造为附加可移动的阶段和莫尔/重建掩模到一个传统的荧光显微镜。在基于活动图像处理的掩模双移动方法下，利用一维掩模进行重建实验 ...更多信息 生物样品的检测技术。从该实验中，已经很好地证实了沿光轴的真实3D分辨率沿着。另一方面，也已经发现，目前的莫尔荧光显微镜是非常敏感的，并导致与莫尔/重建掩模之间的错位的对比度反转。本文对这种效应进行了分析，并提出了相应的校正建议。利用二维掩模进行了重建实验，并在下一阶段（1989年）的研究中证实了真实的三维分辨率。已尝试通过从观察到的槽散焦系列计算3D光谱来评估3D OTF。为了提高光学系统的亮度，引入了相位掩模来代替原来的振幅掩模，虽然超分辨率的确认目前还有待于进一步的研究，但目前的莫尔荧光显微术有望在生物医学领域中得到应用。少

项目成果

T.Ikuta;K.Kishioka: Appl.Opt.

T.Ikuta；K.Kishioka：Appl.Opt。

T. Ikuta and K. Kishioka: "Mask misalignment effect and its correction in moire super resolution microscope (now in preparation)"

T. Ikuta 和 K. Kishioka：“莫尔超分辨率显微镜中掩模错位效应及其校正（正在准备中）”

T.Ikuta and K.Kishioka: "Moire super resolution fluorescent microscopy" appl.Opt.

T.Ikuta 和 K.Kishioka：“莫尔超分辨率荧光显微镜”appl.Opt。

T.Ikuta and K.Kishioka: "Moire super resolution fluorescent microscopy(仮題)" Appl.Opt.

T.Ikuta 和 K.Kishioka：“莫尔超分辨率荧光显微镜（暂定名称）”Appl.Opt。

T.Ikuta and K.Kishioka: "Mask misalignment effect and its correction in moire super resolution microscope(仮題)" Appl.Opt.

T.Ikuta 和 K.Kishioka：“莫尔超分辨率显微镜中掩模错位效应及其校正（暂定名称）”Appl.Opt。