絨毛外トロホブラスト浸潤能へのアクチン・ミオシン発現の関与とその局所・内分泌調節

肌动蛋白和肌球蛋白表达参与绒毛外滋养细胞侵袭能力及其局部和内分泌调节

• 批准号: 18659487
• 负责人: 丸尾 猛
• 金额: $ 2.05万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18659487/
• 关键词: 絨毛外トロホブラスト; 浸潤能; アクチン; ミオシン; 妊娠高血圧症候群; 胎盤

アクチンは脊椎動物組織に6種類のアイソフォームが発現し、アイソフォームには3種類のαアクチン(心筋、骨格筋、平滑筋)、1種類のβアクチン(非筋)、2種類のγアクチン(非筋、平滑筋)が含まれ、筋組織や非筋組織の分化の指標となる。胎盤組織ではβ非筋アクチンがα平滑筋アクチンより多く存在する。一方、ミオシンには筋組織や非筋組織中に複数種のミオシン重鎖アイソフォームが存在する。大動脈平滑筋細胞には3〜4種類のMHCアイソフォームがあり、SM1とSM2という平滑筋タイプMHCアイソフォームと、MIIA(NM)という非筋タイプMHCアイソフォームが存在する。正常末期胎盤に比して妊娠高血圧症候群胎盤では、細胞接着関連分子のfibronectinとその受容体のintegrinα5β1発現は弱く、TIMP-1発現は強い。妊娠高血圧症候群胎盤EVTにおけるアクチンならびにミオシン各種アイソフォーム発現を正常胎盤のそれと比較し、細胞接着関連分子とアクチンならびにミオシンの機能相関として、TIMP-1に焦点を当て検討した。また着床不全の内分泌因子であるプロゲステロンと甲状腺ホルモンのEVTでのアクチンならびにミオシン発現への影響を検討した。さらに、妊娠各期胎盤EVTにおけるアクチンならびにミオシン各種アイソフォームの局在を妊娠極初期(妊娠4-5週)、妊娠初期(妊娠6-12週)、妊娠末期(妊娠37-41週)において、アクチンならびにミオシン各種アイソフォームの局在を特異的モノクロナール抗体を用いて検討した。

6 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 1 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 1 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 1 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 1 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 1 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 1 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 1 species of vertebrate tissues were found, 2 species of vertebrate tissues were found, 3 species of vertebrate tissues were found, 3 species of vertebrate tissues were Placenta tissue is not β-smooth muscle, but α-smooth muscle. There are multiple types of re-locking systems in a single, single, or non-single tissue. Smooth muscle cells in the aorta contain 3 to 4 types of MHC software, SM1, SM2, MIIA (NM), MIIA(NM) and MIIA(NM). The expression of integrinα5β1 and TIMP-1 in the placenta of pregnancy hypertension syndrome is weak and strong, respectively. A comparison of the development of placenta EVT and TIMP-1 in pregnant women with normal placenta and the functional correlation between cell adhesion molecules and EVT. To investigate the effects of endocrine factors on the development of endocrine dysfunction. In addition, placental EVT at all stages of pregnancy is detected by specific antibodies at the early stages of pregnancy (4-5 weeks of pregnancy), early stages of pregnancy (6-12 weeks of pregnancy), and late stages of pregnancy (37-41 weeks of pregnancy).

项目成果

Matrix metalloproteinase (MMP)-1 and MMP-9 promoter polymorphisms are not associated with an increased risk of uterine leiomyomas in a Japanese population

基质金属蛋白酶 (MMP)-1 和 MMP-9 启动子多态性与日本人群子宫肌瘤风险增加无关

• 发表时间: 2006
• 期刊: J Soc Gynecol Investig 13
• 作者: Takemura N;Yoshida S;Kennedy S;Deguchi M;Ohara N;Maruo T
• 通讯作者: Maruo T

Concentration-dependent effects of a selective estrogen receptor modulator raloxifene on proliferation and apoptosis in human uterine leiomyoma cells cultured in vitro

• DOI: 10.1093/humrep/del515
• 发表时间: 2007-05-01
• 期刊: HUMAN REPRODUCTION
• 影响因子: 6.1
• 作者: Liu, Jin;Matsuo, Hiroya;Maruo, Takeshi
• 通讯作者: Maruo, Takeshi

Progesterone and progesterone receptor modulator in uterine leiomyomagrowth: their implication in reproductive health

子宫肌瘤生长中的孕酮和孕酮受体调节剂：对生殖健康的影响

• 发表时间: 2007
• 作者: Maruo T;Matsuo H;Samoto T;Nakago S;Xu Q;Chen W;Wang J;Ohara N
• 通讯作者: Ohara N

Prevention of postpartum hemorrhage by uterotonic agents: comparison of oxytocin and methylergometrine in the management of the third stage of labor

• DOI: 10.1080/00016340600756912
• 发表时间: 2006-11-01
• 期刊: ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA
• 影响因子: 4.3
• 作者: Fujimoto, Miki;Takeuchi, Kyousuke;Maruo, Takeshi
• 通讯作者: Maruo, Takeshi

Selective progesterone receptor modulator asoprisnil induces endoplasmic reticulum stress in cultured human uterine leiomyoma cells

选择性黄体酮受体调节剂阿索普尼诱导培养的人子宫肌瘤细胞内质网应激

• 发表时间: 2007
• 期刊: Am J Physiol Endocrinol Metab 293
• 作者: Xu Q;Ohara N;Liu J;Nakabayashi K;DeManno D;Chwalisz K;Yoshida S;Maruo T
• 通讯作者: Maruo T