Structure analyses of dextran synthases

本文旨在从低聚异麦芽糖糖苷酶和水解酶的晶体结构出发，揭示其底物识别机制。与DGase的晶体结构，异乳寡糖水解酶，提出了水的排水结构在底部的活性口袋。我们研究了该废水中的定点突变对该酶水解活性的影响。水解活性显著降低，但其结构没有任何变化。

We aimed to reveal the substrate recognition mechanism of isomaltooligosaccharide synthases and hydrolases with their crystal structures. With the crystal structure of DGase, isolamtooligosaccharide hydrolase, it is proposed that the water drain structure at the bottom of the active pocket. We study the effect of the site-directed mutagenesis in this water drain on the hydrolysis activity of this enzyme. The hydrolysis activity was decreased significantly without any difference in its structure.