权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

基底膜構築に関与するストレス蛋白質の検索

寻找参与基底膜构建的应激蛋白

基本信息

批准号：
05268221
负责人：
北川泰雄
金额：
$ 1.6万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1993
资助国家：
日本
起止时间：
1993 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05268221/
关键词：
laminin endothelial cell chaperonin basement membrane transport morphogenesis

项目摘要

細胞外基質の主要構成成分であるラミニンは、EHS腫瘍由来のA(400 kDa),B1(230 kDa),B2(210 kDa)の3本のサブユニットによるものを原型とし、各種組織ではこれらのサブユニットの一部または全てがすり変わった組織特異的な異形体が発現している。会合には各鎖の領域IとIIのα-ヘリックス構造が形成する疎水性基及びイオン性基の相互作用が寄与している。細胞内におけるラミニン原型と異形体の合成には特定のサブユニット間会合を選択する機構が考えられる。またラミニンの細胞内輸送においては、成熟した3本鎖ラミニンのみを細胞外へ分泌する選別機構が存在する。本研究では異形体A'B_1B_2を発現するウシ大動脈内皮細胞(BAEC)マウス胎児性ガン細胞F9及びカリニン(kA kB_1 kB_2),K-ラミニン(kA B_1 B_2)を発現するヒト培養皮膚細胞を用いてラミニン構成鎖の会合に寄与するシャペロンを検索した。放射標識後の細胞を細胞膜透過性の架橋剤DSPで処理し、免疫抗体沈降後の電気泳動分析によってシャペロン様物質として検出したところ、BAECでは80,60,50 kDa、F9では100 kDa、ヒト皮膚細胞では90,70,50 kDaのラミニン異形体に特異的なシャペロンの存在が示唆された。BAECを用いた免疫抗体沈降、還元、非還元条件下における二次元電気泳動の結果は、シャペロン様物質が1本のラミニン鎖に多数結合して作用することを示した。また細胞内小胞の酸性化を阻害するモネンシンの存在下ではシャペロン様物質とラミニンの共沈が抑えられた。これらの結果に基き、小胞体中でのラミニンサブユニット間の2本鎖、3本鎖の選択的会合を鎖上の疎水性基との結合と、輸送小胞の酸性化に依存した成熟ラミニンの放出によって調節するシャペロンの作用モデルが考えられた。

The main components of the extracellular matrix are kDa, B1 (230kDa), B2 (210kDa), which are the main components of the whole body of the whole organization. Meet each other in various fields to form the interaction between water-borne groups and sexual groups in different fields. The body of the prototype prototype of the cell has been synthesized and selected by the local government in conjunction with the selection of the local government. There is an increase in the number of extracellular secretions that have been selected by other institutions. In this study, we found that the endothelial cell (BAEC), fetal endothelial cell (BAEC), fetal endothelial cell (F9) and fetal endothelial cell (kA kB_1 kB_2) were detected in this study, and the cells were sent to the cell line in the form of a rendezvous (rendezvous) in the form of an endothelial cell (BAEC), an endothelial cell (BAEC) and a fetal cell (kA kB_1 kB_2). After radiation, the cell membrane permeability scaffold DSP assay, immuno-antibody sedimentation assay, electrophoretic analysis, BAEC antibody 80th, 50th kDa, F9, 100kDa, 90pm, 50th, 50th, 50th, 80th, 80th, 80th, 80th, 80th, 80th, 50th, 50th, 50th, 50th, 50th, 80th, 80th, 50th, 50th, 80th, 80th, 50th, 50th, 50th, 50th, 50th, 50th, 50th, 50th, 50th, 50th, 50th, 6th, 2nd, 6th, 5th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 6th, 1st, 2nd, 6th, 6th, 2nd, 2nd, 6th, 2nd, 6th, 6th, 6th, The results of two-dimensional electrophoretic electrophoresis and the results of two-dimensional electrophoresis under the condition of anti-BAEC immuno-antibody precipitation, immunoglobulin and non-antibody were used to detect the results of two-dimensional electrophoresis and the results of two-dimensional electrophoresis. most of them showed good results in combination with Elisa. In the presence of acidulation, intracellular acidulation, inhibition, inhibition, and co-precipitation, the intracellular acidulation of intracellular cells was acidified and inhibited by the presence of microorganisms. The results of this study were as follows: the basis of the results, the combination of water-based drugs on the rendezvous of the selected materials in the small cells, the combination of water-based drugs, the acidulation of the cells and the dependence on the acidity of the cells. The results showed that there was a significant difference between the two groups in the confluence of the selected materials in the small cells.