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多元的スクリーニング系を用いた未開拓乾燥地生物資源由来の生体機能分子の探索

使用多维筛选系统搜索源自未开发的旱地生物资源的生物功能分子

基本信息

批准号：
18032015
负责人：
繁森英幸
金额：
$ 4.03万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

未開拓乾燥地の生物資源を探索源とし、多元的スクリーニング系を用いて新規機能性物質の探索を行い、生活習慣病の予防薬や治療薬への開発を目的として探索研究を行った。チュニジア原産の4種の植物抽出物について、マウスメラノーマ細胞(B16)を用いた活性試験の結果、Thymelaea hirsutaの抽出物に細胞毒性を示さず、メラニン産生のみを阻害している活性のあることが見い出された。そこで、この活性を指標にして、カラムクロマトグラフィーならびにHPLCを用いて分離・精製し、活性物質を単離した。得られた活性物質について、2次元NMRおよびMS等のスペクトルデータに基づいて解析を行ったところ、4種の既知daphnane型ジテルペンに加えて、2種の新規ジテルペン化合物hirseinAおよびBを見い出した。これらの新規化合物と既知化合物についてB16を用いたメラニン産生阻害活性を調べた結果、側鎖の大きさによって活性が異なることがわかった。一方、寄生植物アメリカネナシカズラより単離し、コーヒー等にも含まれている3,5-di-ο-caffeoylquinic acid(3,5-diCQA)に、アルツハイマー症毒素アミロイドβに対する神経細胞保護作用のあることを見い出した。そこで本研究では3,5-diCQAによる学習記憶障害改善効果を評価するため、アルツハイマー症モデルマウスを用いてモリス水迷路のフラットホームに到着する時間を30日間測定した。その結果、7日目から3,5-diCQA非投与群と比べて投与群の到着時間の短縮が認められ、25日目からは3,5-diCQA投与群の到着時間が著しく短縮された。これらの結果により、3,5-diCQAのようなカフェオイルキナ酸がアルツハイマー発症による学習・記憶障害を改善する効果をin vivoで確認した。

In order to explore the source of biological resources in dry land, multiple biological resources are used to explore the source of biological resources, and to explore the source of biological resources in dry land, the exploration of biological resources, the exploration of sources of biological resources, the exploration of biological resources, the use of new functional materials, the prevention and treatment of diseases and the prevention and treatment of diseases. The plant extract (B16) was used to detect the activity of the plant extract (B16). The cytotoxicity of the extract from the plant (B16) was determined by the results of the bioactivity test, the cytotoxicity test of the extract from the plant, the cytotoxicity of the extract, the cytotoxicity of the extract, the cytotoxicity of the extract and the activity of the extract. "active" and "active" means to separate the essence from the HPLC, and the active substances are isolated from each other. The bioactive substances, NMR compounds, MS, etc., were obtained. The antigens were analyzed. The known Daphnane-type compounds were added, and the compounds hirseinA were isolated. The known compound B16 was used as an inhibitor of biological activity. The results showed that the activity of the known compound B16 was higher than that of the new compound. On the one hand, parasitic plants, parasites, parasitoids, parasitoids, parasite, parasitic plants, parasitic plants, parasitic The purpose of this study is to improve the damage and damage in this study. in this study, the health care system of CQA has been improved in this study. in this study, in this study, we have not been able to improve the damage and damage in this study. in this study, we have not been able to improve the damage. The results show that on the 7th, there is a short period of time between the program and the group, and on the 25th, there is a short period of time between the program and the group. the results show that on the 7th, there is a short period of time between the program and the group. The results showed that there was no significant difference in the results of the study, and the results showed that there was no significant difference in the results of the study. The results showed that the results showed that the symptoms of the disease were improved, and the results were improved. The results showed that the in vivo was confirmed.