New Methods for Directed Molecular Evolution of Novel Protein Functions

新蛋白质功能定向分子进化的新方法

• 批准号: LP0454089
• 负责人: Prof Nicholas Dixon
• 金额: $ 8.55万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-03-17 至 2005-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0454089
• 关键词: New; Methods; Directed; Molecular; Evolution

Novel ribosome-based techniques can be used to carry out test-tube evolution of proteins with new structures and functions. The methods rely on (a) physical association of individual nucleic acid molecules with the particular protein molecules they encode, (b) selection of proteins with new functions, and (c) recovery of the attached genetic code. This project will address several issues that currently limit use of these frontier technologies for evolution of new protein products that have a wide range of practical applications.

基于核糖体的新技术可用于进行具有新结构和功能的蛋白质的试管进化。这些方法依赖于（a）单个核酸分子与它们编码的特定蛋白质分子的物理结合，（B）选择具有新功能的蛋白质，和（c）恢复所连接的遗传密码。该项目将解决目前限制使用这些前沿技术来开发具有广泛实际应用的新蛋白质产品的几个问题。