Molecular Interactions with an antibiotic target in DNA replication

DNA 复制中与抗生素靶标的分子相互作用

• 批准号: DP180100805
• 负责人: Prof Nicholas Dixon
• 金额: $ 37.41万
• 依托单位: University of Wollongong
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-01-01 至 2021-02-07
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP180100805
• 关键词: Molecular; Interactions; antibiotic; target; DNA

This project aims to develop and use new technologies to address mechanistic aspects of anti-bacterial compounds in development, and of the development of resistance to them. The project will focus on the sliding clamp subunit of the bacterial replicative polymerase by studying its association with many other proteins in vitro and in vivo, using novel techniques in solid-state NMR, single-molecule fluorescence and molecular microbiology. The outcomes are expected to be an increased understanding of bacterial DNA replication and mechanisms of antibiotic action and resistance. This project expects to generate new knowledge to assist in combatting antibiotic resistance in Gram-negative bacterial pathogens.

该项目旨在开发和使用新技术来解决抗菌化合物开发中的机械方面问题，以及对它们的耐药性的发展。该项目将利用固体核磁共振、单分子荧光和分子微生物学等新技术，通过研究细菌复制聚合酶的滑动钳子亚单位与许多其他蛋白质在体外和体内的相互作用来研究该亚单位。预计结果将增加对细菌DNA复制以及抗生素作用和耐药性机制的了解。该项目希望产生新的知识，以帮助抗击革兰氏阴性细菌病原体的抗生素耐药性。