Single Molecule Studies of Replisomal Function

最近，用专门的显微镜实时观察DNA分子的复制已经成为可能。这个过程需要在DNA上组装一个复杂的分子机器，然后触发它的功能，并产生关于机器如何工作的精致详细信息。在澳大利亚和美国科学家的这项新合作中，我们将在单个DNA分子上组装这些机器，观察它们是如何工作的，并同时测量机器产生的非常微小的力。这将使澳大利亚科学家获得一项研究分子机器的新技术。

It has recently become possible to watch the copying of DNA molecules with a specialized microscope in real time. This process requires assembly of a complex molecular machine on the DNA, followed by triggering of its function, and yields exquisitely detailed information about how the machine works. In this new collaboration between scientists in Australia and the U.S.A., we will assemble these machines on single DNA molecules, watch how they work and simultaneously measure the very small forces that the machines generate. This will give Australian scientists access to a new technology for studying molecular machines.