Differential localization of CD4ィイD1+ィエD1 T lymphocyte subsets.

A vast majority of CD4T cells infiltrating into gastric mucosa and in the gastric lymph node shows activated memory phenotype，CD45RB I D 1 low文件D1L-selectin I D 1 low文件D1 CD 44 I D 1 high文件D1，in neonataly thymectomized BALB/c mice bearing autoimmune gastritis(AIG)，indicating that these cells are actively involved in this diseasease.CD4 I D1 I D 1 T cells of gastric lymph node expressed both of mRNAs encoding IFN-γand IL-4.However，those infiltrating into gastric mucosa expressed very low level of IL-4mRNA，even though they strongly expressed IFN-γmRNA。Among CD 4 I D 1 I D 1 T cells separated from AIG mice expressing detectable levels of either IFN-γor IL-4 by intracellular staining，only less than one seventh expressed IL-4 and thus most of them expressed IFN-γin gastric mucosa whereas roughly the half and one third expressed IL-4 in gastric node and spleen，respectively.These findings indicate that the Th1cells predominantly infiltrate into autoimmune lesions and Th2cells are mainly resident in the regional lymph node。We further set up an in vitro model system of transendothelial migration using a murine endothelial cell line。F-2.And found that Th1cells in CD4 Th1it D1Tcells separated from lymphoid tissues of AIG mice preferentially passed through the monolayer of endothelial cells while little portion of Th2cells did so.Such different ability of transendothelial migration and localization might explain the dominance of Th1cells destroying the tissue in focal lesion without inhibition by the Th2cells，in spite of both of subsets simultaneously activated in AIG mice and the functions of each T cell subset seem to be mutually exclusive。Using Th1 and Th2cells，taken from normal or TCR transgenic mice and induced in vitro，such differential transendothelial migration was reproduced。Therefore preferential transendothelial migration seems to be a general character of Th1cells。

A vast majority of CD4+ T cells infiltrating into gastric mucosa and in the gastric lymph node shows activated memory phenotype, CD45RBィイD1lowィエD1 L-selectinィイD1lowィエD1 CD44ィイD1highィエD1, in neonataly thymectomized BALB/c mice bearing autoimmune gastritis (AIG), indicating that these cells are actively involved in this disease. CD4ィイD1+ィエD1 T cells of gastric lymph node expressed both of mRNAs encoding IFN-γ and IL-4. However, those infiltrating into gastric mucosa expressed very low level of IL-4 mRNA, even though they strongly expressed IFN-γ mRNA. Among CD4ィイD1+ィエD1 T cells separated from AIG mice expressing detectable levels of either IFN-γ or IL-4 by intracellular staining, only less than one seventh expressed IL-4 and thus most of them expressed IFN-γ in gastric mucosa whereas roughly the half and one third expressed IL-4 in gastric lymph node and spleen, respectively. These findings indicate that the Th1 cells predominantly infiltrate into autoimmune lesions and Th2 cells are mainly resident in the regional lymph node. We further set up an in vitro model system of transendothelial migration using a murine endothelial cell line. F-2. and found that Th1 cells in CD4ィイD1+ィエD1 T cells separated from lymphoid tissues of AIG mice preferentially passed through the monolayer of endothelial cells while little portion of Th2 cells did so. Such different ability of transendothelial migration and localization might explain the dominance of Th1 cells destroying the tissue in focal lesion without inhibition by the Th2 cells, in spite of both of subsets simultaneously activated in AIG mice and the functions of each T cell subset seem to be mutually exclusive. Using Th1 and Th2 cells, taken from normal or TCR transgenic mice and induced in vitro, such differential transendothelial migration was reproduced. Therefore preferential transendothelial migration seems to be a general character of Th1 cells.