A flow cytometry suite for multiscale biosorting

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The proposal aims to establish a state-of-the-art flow cytometry suite that will allow for the first time in Australia at one site, the separation of the broadest range of sizes of biological samples from viruses, to plant seeds and animal embryos. The flow cytometry suite will include three cutting edge flow cytometers (i) a 2 laser Union Biometrica BioSorter large particle flow cytometer for large objects (eg embryos); (ii) a 5 laser BD Biosciences FACSAria Fusion cell sorter with FSC PMT for small to mid-size objects (eg cells), (iii) a 2 laser NanoFCM flow nanoanalyzer for the smallest biological objects (eg viruses, vesicles). These new broad capabilities will support research in agricultural, veterinary and microbiological sciences.