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Osmoreceptorand arugihine vasotocin I n chicken

鸡渗透压感受器和阿鲁吉辛催产素

基本信息

批准号：
12660253
负责人：
SAITO Noboru
金额：
$ 2.3万
依托单位：
NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660253/
关键词：
chicken aquaporm osmoregulation cloning sequencing

项目摘要

It is well known about the idea that antidunesis mav be contmlled hv cells in the, hypothalamus acting as 'vesicular osmometer'. There has been considerable debate on the identity and sensitivity of such cells, focusing mainly on the neurons located in osmosensitive nuclei. The aniannrin l(AQR) family of membrane bound water transporting channels is expressed in a variety of origanisms. Channel- forming integral protein of 28 kDa was the first aquaporinto be jdentified and characterized AQP4 is a newly discoberedmembrane of this family. AQP4 iswidely distri but expression of brain cells through AQP4 cDNA did not yet identified. The purpose of this study was the cloning of AQP4 cDNA from the birds and understood the physiological function of AQP4 in related with osmoreception in birds,. The total RNA was extracted from one-day old chick brain and was reverse translated into cDNA was used for PCR with the primenrs designed from mammmalian AQP4 cDNA scquence. The PCR product was subcloned … More sith pGEM-T easy vector, and was sequenced. Five one-day old chicks were dehydrated for on day and their brain was removed. IN control chicks, five brains were removed without the dehydration treatment. The total RNA was extracted from the chick brain and was reverse-translated into cDNA. PCR was performed with chicken AQP4 primers. PCR products was 376bp. Using BLAST program, this PCR products was searchedthe homologous gene. The results showed that this PCR product was highoy similar to AQP4 cDNA sequence of mammalian. For example, between human AQP4 and this PCR product, identity was 312/368(86%), and Score was 333 bits, and between mouse AQP4 and this PCR product, identity was 312/368(86%), and Score was 303 bits. These results highly suggeste that this PCR product might be chiken AQP4. But, this is still partial sequence, and then we are working on the cloning of full sequence of this gene. With RT-PCR, the AQP4 mRNA expression in the dehydrated chicken brain was slightly higher than those in control brains. This result suggest that AQP4 may be relate with osmoregulation. Less

下丘脑中的hv细胞起着“囊泡渗透压计”的作用，这一观点是众所周知的。关于这些细胞的身份和敏感性有相当多的争论，主要集中在位于神经元敏感核中的神经元上。膜结合水转运通道的aniannrin I（AQR）家族以多种起源表达。28 kDa的通道形成蛋白是第一个被鉴定和鉴定的水通道蛋白，AQP 4是该家族中新发现的一种膜。AQP 4分布广泛，但通过AQP 4 cDNA表达的脑细胞尚未确定。本研究的目的是克隆鸟类AQP 4的cDNA，了解AQP 4在鸟类体内与呼吸感受相关的生理功能。从1日龄鸡脑组织中提取总RNA，反转录成cDNA，用已克隆的AQP 4 cDNA序列设计引物进行PCR。将PCR产物进行亚克隆 ...更多信息构建pGEM-T easy载体，并进行测序。将5只1日龄雏鸡脱水1天，取出其脑。在对照组中，5只鸡的大脑被取出，没有脱水处理。从鸡脑中提取总RNA并反向翻译成cDNA。用鸡AQP 4引物进行PCR。PCR扩增产物为376 bp。用BLAST程序对该PCR产物进行同源基因搜索.结果表明，该PCR产物与哺乳动物AQP 4 cDNA序列高度相似。例如，在人AQP 4和该PCR产物之间，同一性为312/368（86%），Score为333位，而在小鼠AQP 4和该PCR产物之间，同一性为312/368（86%），Score为303位。这些结果高度证明该PCR产物可能是鸡AQP 4。但是，这仍然是部分序列，然后我们正在克隆这个基因的全序列。RT-PCR结果显示，脱水鸡脑中AQP 4 mRNA的表达量略高于对照组。提示AQP 4可能参与了肾脏的调节。少