The study of molecular marker expression in evaluation for the malignancy potential of colorectal cancer

分子标志物表达在结直肠癌恶性潜能评估中的研究

• 批准号: 16591357
• 负责人: SAITO Noboru
• 金额: $ 1.02万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591357/
• 关键词: colorectal cancer; adhesion molecule; cell growth factor; molecular marker; laminin; TGF-β; HGF; EGF; 細胞接着; 肝転移; 肺転移; 多変量解析; 細胞増殖; fibronectin

We investigated the molecular marker expression in evaluation for the maliginancy potential of colorectal cancer.1. HGF(hepatocyte growth factor), EGF(epidermal growth factor)(1) Serological analysis demonstrated that preoperative serum HGF and EGF level was significantly higher in the cancer group than in the control group (p < 0.0001).(2) Immunohistochemical analysis demonstrated the strong expression and colocalization of HGF, c-Met and HGF in the cancer cells.2. TGF-β(1) In immunohistological staining, the positive rate became higher in lymph-node metastases and in progressing invasion into the vascular system.(2) In serological examination, a significant difference was found between cancer cases and non-cancer cases, and TGF-β1 level rose with progressing liver metastasis and venous invasion.3. Laminin(1) Mean serum laminin in subjects with colon cancer was 606.3±260.2 ng/ml, significantly higher than that of 258.0±92.0 ng/ml in normal controls (p<0.0001).(2) The positive rate was higher at 89.3% for laminin vs 38.0% for CEA and 19.5% for CA19-9.(3) Mean serum laminin in subjects with hepatic metastases was 668.0±274.7 ng/ml, significantly higher than that of 585.2±252.5 ng/ml in subjects without hepatic metastases (p=0.0472).(4) Survival rates were significantly lower in the high (【greater than or equal】520 ng/ml) than in the low laminin group (<350 ng/ml) (P=0.0451). Univariate and multivariate analysis, using Cox' s proportional hazard regression model, showed serum laminin is an independent prognostic factor in colorectal cancer, along with hepatic, pulmonary and peritoneal metastases.

我们研究了分子标志物的表达在评估结直肠癌恶性潜能中的作用。HGF（肝细胞生长因子）、EGF（表皮生长因子）(1)血清学分析显示，术前肿瘤组血清HGF、EGF水平明显高于对照组（p < 0.0001）。(2)免疫组化分析显示HGF、c-Met和HGF在肿瘤细胞中有较强的表达和共定位。TGF-β(1)免疫组织染色中，淋巴结转移和进展性血管系统浸润的阳性率增高。(2)血清学检查中，癌组与非癌组差异有统计学意义，TGF-β1水平随肝转移进展及静脉浸润而升高。(1)结肠癌患者的平均血清层粘连蛋白为606.3±260.2 ng/ml，显著高于正常对照组的258.0±92.0 ng/ml （p<0.0001）。(2)层粘连蛋白的阳性率为89.3%，CEA为38.0%，CA19-9为19.5%。(3)肝转移组血清层粘连蛋白平均值为668.0±274.7 ng/ml，显著高于无肝转移组的585.2±252.5 ng/ml （p=0.0472）。(4)层粘连蛋白高组（【大于等于】520 ng/ml）的存活率显著低于低组（<350 ng/ml） （P=0.0451）。单因素和多因素分析，采用Cox比例风险回归模型，显示血清层粘连蛋白与肝、肺和腹膜转移一起是结直肠癌的独立预后因素。

项目成果

ヒト大腸癌悪性度評価におけるMMP-2,9, E-cadherin発現の臨床的意義

MMP-2,9、E-cadherin表达在人结直肠癌恶性程度评价中的临床意义

• 发表时间: 2004
• 期刊: 東京女子医科大学雑誌 74巻 1号
• 作者: 中澤 哲;齋藤 登;亀岡信悟
• 通讯作者: 亀岡信悟

大腸癌悪性度評価におけるTGF-β1発現の臨床的意義

TGF-β1表达在结直肠癌恶性程度评估中的临床意义

• 发表时间: 2005
• 期刊: 日本大腸肛門病学会雑誌 58巻 7号
• 作者: 大幸和加子;齋藤 登;亀岡信悟
• 通讯作者: 亀岡信悟

Clinical significance of matrix metalloproteinase(MMP)-2,-9 and E-cadherin expression for assessing the spreading of Human colorectal cancer

基质金属蛋白酶(MMP)-2、-9和E-钙粘蛋白表达对评估人结直肠癌扩散的临床意义

• 发表时间: 2004
• 期刊: Journal of Tokyo Women' s Medical University Vol.58 No.74
• 作者: Satoru Nakazawa;Noboru Saito;Shingo Kameoka
• 通讯作者: Shingo Kameoka

Differential alternative splicing expressions of telomerase reverse transcriptase in gastrointestinal cell lines.

• DOI: 10.3892/or.11.1.127
• 发表时间: 2004
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: K. Nagao;K. Katsumata;Y. Aizawa;N. Saito;Hiroyuki Hirata;Hiroshi Sasaki;Shigeki Yamamoto;K. Hikiji;T. Koiwa;H. Hisatomi

Clinical Significance of TGF-β1 Expression in Evaluation of the Malignancy of Colorectal Cancer

TGF-β1表达在结直肠癌恶性程度评估中的临床意义

• 发表时间: 2005
• 期刊: Journal of Japan Society of Coloproctology Vol.58 No.7
• 作者: Wakako Daiko;Noboru Saito;Shingo Kameoka
• 通讯作者: Shingo Kameoka