Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid

卵泡液体外培养过程中氧张力和卵泡细胞对猪卵母细胞成熟和受精的影响

• 批准号: 19580332
• 负责人: NAGAI Takashi
• 金额: $ 2.75万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580332/
• 关键词: ブタ; 体外成熟; 体外受精; 卵胞液; 胚盤胞期胚; 体外発生; 未成熟卵子; 卵丘細胞; 静置培養; 回転培養

The objective was to investigate the effects of oxygen tension (5 or 20% O2) and follicle cells (FC) during in vitro maturation of porcine oocytes in only porcine follicular fluid, using static (S group) and non-static (rotating)(R group) culture systems, on their nuclear maturation and subsequent in vitro fertilization and development. Both -FC/S and +FC/R culture systems supported meiotic competence, irrespective of oxygen tension. However, the +FC/R culture system may be superior to the -FC/S culture system for promoting in vitro fertilization and development of in vitro matured porcine oocytes.

目的是使用静态（S组）和非静态（旋转）（R组）培养系统，研究仅在猪卵泡液中的猪卵母细胞体外成熟过程中氧张力（5％或20％O2）和卵泡细胞（FC）对其核成熟以及随后的体外受精和发育的影响。无论氧张力如何，-FC/S 和+FC/R 培养系统都支持减数分裂能力。然而，+FC/R培养系统在促进体外受精和体外成熟猪卵母细胞发育方面可能优于-FC/S培养系统。

项目成果

Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid

• DOI: 10.1016/j.theriogenology.2009.11.013
• 发表时间: 2010-04-15
• 期刊: THERIOGENOLOGY
• 影响因子: 2.8
• 作者: Agung, B.;Piao, Y.;Nagai, T.
• 通讯作者: Nagai, T.