Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid

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The objective was to investigate the effects of oxygen tension (5 or 20% O2) and follicle cells (FC) during in vitro maturation of porcine oocytes in only porcine follicular fluid, using static (S group) and non-static (rotating)(R group) culture systems, on their nuclear maturation and subsequent in vitro fertilization and development. Both -FC/S and +FC/R culture systems supported meiotic competence, irrespective of oxygen tension. However, the +FC/R culture system may be superior to the -FC/S culture system for promoting in vitro fertilization and development of in vitro matured porcine oocytes.