Neuron-Like Differentiation and Selective Ablation of Undifferentiated Embryonic Stem Cells Containing Suicide Gene with Oct-4 Promoter

含有Oct-4启动子自杀基因的未分化胚胎干细胞的神经元样分化和选择性消融

• 批准号: 19592012
• 负责人: HARA Akira
• 金额: $ 2.41万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19592012/
• 关键词: 胚性肝細胞; ES細胞; 神経組織再生; 奇形腫; 自殺遺伝子; thymidine kinase; ganciclovir human; 胚性幹細胞; ganciclovir; human

研究成果が5編の論文として国際雑誌に掲載された。これら論文には、基礎研究としての海馬神経細胞、眼球光彩色素細胞の研究も含まれる。OCT-4遺伝子プロモーター下流に自殺遺伝子thymidine kinase を挿入したES細胞(OCT-TK-ES細胞)を3次元培養し、ganciclovir投与により選択的に未分化ES細胞の増殖を抑制し、神経分化蛋白発現の変化を検索し、ganciclovir投与により未分化細胞成分のみが除去され、神経分化を示すことが示された。

The research results are published in five parts of the paper and published in international journals. This paper focuses on basic research and research on hippocampal neurons and retinal pigment cells. The introduction of OCT-4 gene promoter thymidine kinase into ES cells (OCT-TK-ES cells) in three-dimensional culture, ganciclovir administration to selected undifferentiated ES cells inhibited proliferation, the expression of neurodifferentiation proteins, ganciclovir administration to undifferentiated cell components were detected.

项目成果

Neuron-like differentiation and selective ablation of undifferentiated embryonic stem cells containing suicide gene with Oct-4 promoter

• DOI: 10.1089/scd.2007.0235
• 发表时间: 2008-08-01
• 期刊: STEM CELLS AND DEVELOPMENT
• 影响因子: 4
• 作者: Hara, Akira;Aoki, Hitomi;Mori, Hideki
• 通讯作者: Mori, Hideki

Marked increases in hippocampal neuron indoleamine 2, 3-dioxygenase via IFN-γ-independent pathway following transient global ischemia in mouse

• DOI: 10.1016/j.neures.2008.12.003
• 发表时间: 2009-03-01
• 期刊: NEUROSCIENCE RESEARCH
• 影响因子: 2.9
• 作者: Hoshi, Masato;Saito, Kuniaki;Seishima, Mitsuru
• 通讯作者: Seishima, Mitsuru

Iris as a recipient tissue for pigment cells: Organized in vivo differentiation of melanocytes and pigmented epithelium derived from embryonic stem cells in vitro

• DOI: 10.1002/dvdy.21656
• 发表时间: 2008-09-01
• 期刊: DEVELOPMENTAL DYNAMICS
• 影响因子: 2.5
• 作者: Aoki, Hitomi;Hara, Akira;Kunisada, Takahiro
• 通讯作者: Kunisada, Takahiro

Transplantation of melanocytes into iris Method for iris repigmentation

将黑素细胞移植到虹膜中进行虹膜再色素沉着的方法

• 发表时间: 2008
• 期刊: Transplantation 85
• 作者: Akira Hara;Hitomi Aoki;Hitomi Aoki;Hitomi Aoki
• 通讯作者: Hitomi Aoki

Localization and spatiotemporal expression of IDO following transient forebrain ischemia in gerbils

• DOI: 10.1016/j.brainres.2008.02.067
• 发表时间: 2008-06-27
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Taguchi, Ayako;Hara, Akira;Mori, Hideki
• 通讯作者: Mori, Hideki