Investigation of signal correlation and integration in ICC pacemaking

ICC 起搏信号相关性和集成的研究

• 批准号: 20390198
• 负责人: NAKAYAMA Shinsuke
• 金额: $ 11.9万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390198/
• 关键词: 高ストレス社会; 超高齢化; Cajal細胞; 消化管

(1) First, for investigation of molecular basis, we established procedures to collect ICC from the murine gut by using a flow cytometry with fluorescent antibodies to c-KIT receptors. In collected ICC, PT-PCR detected expression of several important genetic products for Ca oscillations, such as Ca-activated Cl channels(Ano1) and STIM, which acts as a Ca sensor in intracellular Ca stores.(2) Next, using arrayed microelectrodes with low impedance, we successfully characterized spatial properties of the propagation of ICC pacemaker potentials in a small area of 1mm2. Also, we observed that serotonin(5-HT) potentiated both electric and Ca activities in ICC.(3) Furthermore, in experiments of individual animals, oral administrations of 5-HT-related drugs altered behavioral patterns of mice lacking 5-HT transporters for a long time(more than a week), compared to wild-type mice.

(1)首先，为了研究分子基础，我们建立了通过使用c-KIT受体的荧光抗体的流式细胞术从小鼠肠道收集ICC的程序。在收集的ICC中，PT-PCR检测到几种重要的钙振荡基因产物的表达，如钙激活的氯通道（Ano 1）和STIM，其作为细胞内钙储存的钙传感器。(2)接下来，使用低阻抗的阵列微电极，我们成功地表征了ICC起搏电位在1 mm 2的小区域内传播的空间特性。此外，我们还观察到5-羟色胺（5-HT）增强ICC的电活动和钙活动。(3)此外，在单个动物的实验中，与野生型小鼠相比，口服5-HT相关药物可以长期（一周以上）改变缺乏5-HT转运蛋白的小鼠的行为模式。

项目成果

Assessment of gastric emptying and duodenal motility upon ingestion of liquid meal using rapid magnetic resonance imaging

使用快速磁共振成像评估摄入液体膳食后的胃排空和十二指肠蠕动

• 发表时间: 2012
• 期刊: Experimental Physiology
• 影响因子: 2.7
• 作者: Teramoto H;Shimizu T;Yogo H;Nishimiya Y;Hori S;Kosugi T;Nakayama S
• 通讯作者: Nakayama S

Assessment of gastric motility by magnetic resonance imaging in patients with enteral nutrition

磁共振成像评估肠内营养患者胃动力

• 发表时间: 2009
• 期刊: Journal of American Geriatrics Society
• 作者: Teramoto H.;et al.
• 通讯作者: et al.

Microelectrode array evaluation of gut pacemaker activity in wild-type and W/Wv mice

• DOI: 10.1016/j.bios.2009.06.006
• 发表时间: 2009-09-15
• 期刊: BIOSENSORS & BIOELECTRONICS
• 影响因子: 12.6
• 作者: Nakayama, Shinsuke;Ohishi, Ryotaro;Hirose, Kenzo
• 通讯作者: Hirose, Kenzo

Pulse-Driven Magnetoimpedance Sensor Detection of Cardiac Magnetic Activity

• DOI: 10.1371/journal.pone.0025834
• 发表时间: 2011-10-12
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Nakayama, Shinsuke;Sawamura, Kenta;Uchiyama, Tsuyoshi
• 通讯作者: Uchiyama, Tsuyoshi

セロトニントランスポーターの消化管ペースメーカー活動への作用

血清素转运蛋白对胃肠起搏器活性的影响

• 发表时间: 2010
• 作者: 谷口瑞毅;劉(武川)紅年;中山晋介
• 通讯作者: 中山晋介