Development of a temporally regulable gene expression system in ascidian embryos

海鞘胚胎时间调控基因表达系统的开发

• 批准号: 20770183
• 负责人: WADA Shuichi
• 金额: $ 2.75万
• 依托单位: Nagahama Institute of Bio-Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770183/
• 关键词: 遺伝子発現調節; 熱ショック応答; 転写制御領域; 誘導発現系

So far there was no inducible gene expression system in the ascidian Ciona intestinalis. We developed a heat shock-inducible gene expression system based on the cis-regulatory region of the Ciona HSP70 family gene Ci-HSPA1/6/7-like. This system allowed temporally controlled induction of gene expression in ascidian embryos. We also showed that heat shock response of Ci-HSPA1/6/7-like is mediated by the heat shock transcription factor.

目前还没有在海鞘中发现可诱导的基因表达体系。我们基于Ciona HSP70家族基因Ci-HSPA1/6/7-like的顺式调控区构建了热休克诱导基因表达系统。该系统允许暂时控制海鞘胚胎基因表达的诱导。我们还发现，Ci-HSPA1/6/7-like的热休克反应是由热休克转录因子介导的。

项目成果

Stress response in the ascidian Ciona intestinalis: transcriptional profiling of genes for the heat shock protein 70 chaperone system under heat stress and endoplasmic reticulum stress

• DOI: 10.1007/s12192-009-0133-x
• 发表时间: 2010-03-01
• 期刊: CELL STRESS & CHAPERONES
• 影响因子: 3.8
• 作者: Fujikawa, Tetsuya;Munakata, Takeo;Wada, Shuichi
• 通讯作者: Wada, Shuichi