Development of novel diagnostic marker targeting embryonic stem cell replication factors in lung cancers

在这项研究中，通过靶向胚胎干细胞（ESC）的可重复性和/或自我复制所必需的分子，我们旨在开发肺特异性肿瘤标志物。针对Nanog和SALL 4的siRNA的转导导致显著的生长抑制。癌细胞中的SALL 4抑制通过在G1和早期S期的细胞周期停滞引发增殖灾难，导致尺寸增大而没有细胞分裂。在临床样本中，Nanog和SALL 4在癌部分中的表达显示出1000倍和50倍的增加，并且这种表达的改变已经在IA期被发现。ROC曲线分析Nanog和SALL 4基因的敏感性分别为80%（特异性93%）和85%（特异性93%）。这些结果表明，ESC复制因子，特别是SALL 4，在肺癌细胞的增殖中起着至关重要的作用，并且可以作为支持肺癌诊断的标记物，即使在早期临床阶段。

In this study, by targeting the molecules essential in the reproducibility and/or self-replication of embryonic stem cells(ESC), we aimed to develop lung-specific tumor markers. Transduction of siRNAs against Nanog and SALL4 resulted in remarkable growth inhibition. SALL4 inhibition in cancer cells triggered proliferation catastrophe via cell cycle arrest at G1 and early S-phase, leading size enlargement without cell division. In clinical samples, Nanog and SALL4 expression in cancerous part revealed to 1, 000-and 50-fold increase, and this alteration of the expression was found already at stage IA. ROC analysis of Nanog and SALL4 gene resulted in the sensitivity of 80%(specificity of 93%) and the sensitivity of 85%(specificity of 93%). These findings suggested that ESC-replication factor, especially SALL4, plays an crucial role in the proliferation of lung cancer cells and could be applicable as the markers to support the diagnosis of lung cancers even at early clinical stages.