Establishment of diagnosis of influenza C virus infection

丙型流感病毒感染诊断的建立

• 批准号: 21591380
• 负责人: MATSUZAKI Yoko
• 金额: $ 2.58万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591380/
• 关键词: C型インフルエンザ; 迅速診断法

Monoclonal antibodies were produced as the influenza C virus-specific antibodies for rapid diagnosis of influenza C virus infection and the real-time PCR assay for influenza C virus was newly developed. Respiratory specimens from children were examined for the presence of influenza C virus by cell culture and real-time PCR. Taking the cell culture results as the gold standard, the sensitivity, specificity, positive predictive value and negative predictive value for the real-time PCR were found to be 100%, 98.5%, 66.7% and 100%, respectively. The mean viral load in specimens that were positive by cell culture was significantly high than those in specimens that were negative by cell culture. The real-time PCR assay established here can be used as a sensitive method for diagnosing influenza C virus infection

制备了丙型流感病毒特异性单克隆抗体，用于快速诊断丙型流感病毒感染，并开发了丙型流感病毒的实时PCR检测方法。通过细胞培养和实时PCR检测儿童呼吸道标本中是否存在丙型流感病毒。以细胞培养结果为金标准，实时荧光PCR检测的敏感性、特异性、阳性预测值和阴性预测值分别为100%、98.5%、66.7%和100%。细胞培养阳性标本的平均病毒载量显著高于细胞培养阴性标本。建立的实时荧光定量PCR方法可作为诊断丙型流感病毒感染的敏感方法

项目成果

Endemicity of human metapneumovirus subgenogroups A2 and B2 in Yamagata, Japan, between 2004 and 2009

• DOI: 10.1111/j.1348-0421.2010.00253.x
• 发表时间: 2010-10-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Mizuta, Katsumi;Abiko, Chieko;Ahiko, Tadayuki
• 通讯作者: Ahiko, Tadayuki

A two-year survey of the oseltamivir-resistant influenza A(H1N1) virus in Yamagata, Japan and the clinical effectiveness of oseltamivir and zanamivir.

• DOI: 10.1186/1743-422x-7-53
• 发表时间: 2010-03-05
• 期刊: Virology journal
• 影响因子: 4.8
• 作者: Matsuzaki Y;Mizuta K;Aoki Y;Suto A;Abiko C;Sanjoh K;Sugawara K;Takashita E;Itagaki T;Katsushima Y;Ujike M;Obuchi M;Odagiri T;Tashiro M
• 通讯作者: Tashiro M

Saffold Cardiovirus Infection in Children Associated With Respiratory Disease and Its Similarity to Coxsackievirus Infection

• DOI: 10.1097/inf.0b013e31821608a8
• 发表时间: 2011-08-11
• 期刊: PEDIATRIC INFECTIOUS DISEASE JOURNAL
• 影响因子: 3.6
• 作者: Itagaki, Tsutomu;Abiko, Chieko;Matsuzaki, Yoko
• 通讯作者: Matsuzaki, Yoko

Detection and quantification of influenza C virus in pediatric respiratory specimens by real-time PCR and comparison with infectious viral counts

• DOI: 10.1016/j.jcv.2012.02.012
• 发表时间: 2012-06-01
• 期刊: JOURNAL OF CLINICAL VIROLOGY
• 影响因子: 8.8
• 作者: Matsuzaki, Yoko;Ikeda, Tatsuya;Hongo, Seiji
• 通讯作者: Hongo, Seiji

Palmitoylation of CM2 is dispensable to influenza C virus replication

• DOI: 10.1016/j.virusres.2011.02.013
• 发表时间: 2011-04-01
• 期刊: VIRUS RESEARCH
• 影响因子: 5
• 作者: Muraki, Yasushi;Okuwa, Takako;Ohara, Yoshiro
• 通讯作者: Ohara, Yoshiro