Expression and functional analysis of FAM107 family proteins as a new molecular marker for the onco-hyperthermia therapy

我们发现了一个独特的分子FAM107B，一种新的热休克诱导蛋白，并将其命名为HITS。另一个FAM107家族蛋白FAM107A （TU3A/DRR1）作为候选肿瘤抑制基因吸引了肿瘤学家。与FAM107A类似，HITS表达缺失在多器官癌症中也很常见，并与肿瘤生长有关，这与其他热休克蛋白（HSPs）如HSP70和HSP90的致癌活性形成对比。由于HITS表达的下调在不同器官中具有组织病理类型和临床分期特异性，因此HITS可以作为诊断各种癌症的有用标记。由于HITS和FAM107A都在神经元组织中强烈表达，我们也研究了阐明神经元的功能。HITS显著抑制神经突生长和细胞迁移，而FAM107A则没有明显的抑制作用。这些结果表明FAM107家族蛋白参与肿瘤发生和神经发生，并具有独特的功能。

We have identified a unique molecule FAM107B, a novel heat-shock-inducible protein, and designated it HITS. The other FAM107 family protein, FAM107A (TU3A/DRR1), has attracted oncologists as a candidate tumor suppressor gene. Similar to FAM107A, loss of HITS expression is commonly observed in cancers of multiple organs and involved in tumor growth, which contrasts with oncogenic activities of other heat-shock proteins (HSPs) such as HSP70 and HSP90. Because downregulation of HITS expression was marked by histopathological-type and clinical-stage-specific manners in distinct organs, HITS can be a useful marker for diagnosis in various cancers.Since both HITS and FAM107A expressed strongly in neuronal tissues, we also investigated to elucidate neuronal functions. HITS significantly inhibits neurite outgrowth and cell migration, while FAM107A does not so much.These results suggest FAM107 family proteins are involved both in oncogenesis and neurogenesis with distinctive functions.