Mechanism of Cytomegalovirus reactivation after organ transplantation

器官移植后巨细胞病毒再激活的机制

• 批准号: 23591876
• 负责人: TANAKA Kazuo
• 金额: $ 3.16万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591876/
• 关键词: サイトメガロウイルス; ウイルス再活性化; 臓器移植

Aim of this study is to clarify the mechanism of cytomegalovirus (CMV) reactivation after organ transplantation. For this purpose, we developed in vitro and in vivo CMV reactivation models. In in vitro model, two macrophage-derived cell lines were used, and then the cell differentiation and acetylation of histone in CMV infected cells were demonstrated to be critical in CMV reactivation. In in vivo model, we demonstrated the CMV reactivation in the milk of breast-feeding female mice. Interestingly, reactivated CMV titers in the breast milk was much higher in syngeneic pregnancy than those in allogeneic pregnancy, suggesting that allogeneic stimuli is unnecessary in CMV reactivation.

这项研究的目的是阐明器官移植后巨细胞病毒（CMV）重新激活的机理。为此，我们开发了体外和体内CMV重新激活模型。在体外模型中，使用了两种巨噬细胞衍生的细胞系，然后证明了CMV感染细胞中组蛋白的细胞分化和乙酰化在CMV重新激活中至关重要。在体内模型中，我们证明了母乳喂养雌性小鼠的牛奶中的CMV重新激活。有趣的是，在合成妊娠中，母乳中的重新激活的CMV滴度比同种异体妊娠中的CMV滴度要高得多，这表明同种异体刺激是CMV重新激活的不必要的。