Mechanism of Cytomegalovirus reactivation after organ transplantation

器官移植后巨细胞病毒再激活的机制

• 批准号: 23591876
• 负责人: TANAKA Kazuo
• 金额: $ 3.16万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591876/
• 关键词: サイトメガロウイルス; ウイルス再活性化; 臓器移植

Aim of this study is to clarify the mechanism of cytomegalovirus (CMV) reactivation after organ transplantation. For this purpose, we developed in vitro and in vivo CMV reactivation models. In in vitro model, two macrophage-derived cell lines were used, and then the cell differentiation and acetylation of histone in CMV infected cells were demonstrated to be critical in CMV reactivation. In in vivo model, we demonstrated the CMV reactivation in the milk of breast-feeding female mice. Interestingly, reactivated CMV titers in the breast milk was much higher in syngeneic pregnancy than those in allogeneic pregnancy, suggesting that allogeneic stimuli is unnecessary in CMV reactivation.

本研究的目的是阐明器官移植后巨细胞病毒（CMV）再激活的机制。为此，我们开发了体外和体内CMV再激活模型。在体外模型中，使用两种巨噬细胞来源的细胞系，然后证明细胞分化和组蛋白乙酰化在CMV感染的细胞中是CMV再激活的关键。在体内模型中，我们证明了哺乳期雌性小鼠乳汁中的CMV再激活。有趣的是，在母乳中再激活的CMV滴度在同基因妊娠中比在异基因妊娠中高得多，这表明在CMV再激活中异基因刺激是不必要的。