Development of advanced in vivo EMT imaging system and its application for the study of cancer metastasis

为了在体内观察上皮间充质转化(EMT)，我试图通过EMT特异性启动子和双分子荧光互补(BIFC)方法相结合的方法来开发EMT的荧光探针。我使用LZA和LZB在细胞中证明了BIFC方法在体内有效。此外，我在活体小鼠体内建立了MKO、MCherry、CFP和Venus的体内成像系统。最后，我对EMT相关启动子进行了筛选，结果表明TWIST和蜗牛启动子是很好的候选启动子。

In order to visualize Epithelial to Mesenchymal Transition (EMT) in vivo, I tried to develop a fluorescent probe for EMT by combination ofEMT-specific promoters and a Bimolecular Fluorescence Complementation (BiFC) method. I showed that a BiFC method worked in vivo using LZA and LZB in cells. Moreover, I established an in vivo imaging system for mKO, mCherry, CFP and Venus in living mice. Finally, I screened EMT related promoters and showed that promoters for twist and snail are good candidate for the system.