Development of advanced in vivo EMT imaging system and its application for the study of cancer metastasis

为了可视化上皮到间充质转化（EMT）在体内的情况，我试图通过结合EMT特异性启动子和双分子荧光互补（BiFC）方法开发一种EMT荧光探针。我证明了在细胞中使用LZA和LZB的bic方法在体内有效。此外，我还建立了mKO、mCherry、CFP和Venus在活鼠体内的成像系统。最后，对EMT相关启动子进行筛选，发现twist和snail启动子是该系统的良好候选启动子。

In order to visualize Epithelial to Mesenchymal Transition (EMT) in vivo, I tried to develop a fluorescent probe for EMT by combination ofEMT-specific promoters and a Bimolecular Fluorescence Complementation (BiFC) method. I showed that a BiFC method worked in vivo using LZA and LZB in cells. Moreover, I established an in vivo imaging system for mKO, mCherry, CFP and Venus in living mice. Finally, I screened EMT related promoters and showed that promoters for twist and snail are good candidate for the system.