New method of differential diagonosis and tailor- made treatmentfor hepatocellular carcinoma by antisense RNA

关于肝细胞癌发生的分子机制，目前尚无定论。最近的研究表明，具有信使RNA互补序列的天然反义转录本具有稳定和沉默信使RNA等重要功能。我们研究了自然反义转录物(NAT)在人肝细胞癌中的调节，以了解它们可能参与肝细胞癌的发生。与非癌肝组织相比，我们发现在肝细胞癌组织中表达上调和下调。我们还重点研究了与丙型肝炎病毒相关的肝细胞癌，并进行了评估。反义转录产物在肝细胞癌和非癌组织中的表达存在差异。分析正义和反义表达谱与肝细胞癌组织学模糊的关系。我们明确了NAT在肝细胞癌组织学模糊中的不同表达。

The molecular mechanisms of hepatocellular carcinoma (HCC) initiation remain mclear. Recent reports have demonstrated that natural antisense transcripts with complementary sequences to messenger RNA have important functions such as the stabilization and silencing of mRNA. We innestigated the regulation of natural antisense transcripts (NATs) in human HCC for their possible involvement in HCCdevelopment. We identified as up- and down-regulated in HCC tissues, compared to non-cancerous liver tissue. We also focused on HCV-associated HCCs and evaluated. Differential expression of antisense transcripts was observed between HCC and non-cancerous tissue. The sense and antisense expression profiles were analyzed with regard to histological faindings of HCC. We clarifeid different expression of NATs among the histological faindings of HCC.