New method of differential diagonosis and tailor- made treatmentfor hepatocellular carcinoma by antisense RNA

反义RNA肝细胞癌鉴别诊断及个体化治疗新方法

• 批准号: 23650611
• 负责人: OHKOHCHI Nobuhiro
• 金额: $ 2.33万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650611/
• 关键词: バイオマーカー; アンチセンスRNA; 肝癌; スクリーニング

The molecular mechanisms of hepatocellular carcinoma (HCC) initiation remain mclear. Recent reports have demonstrated that natural antisense transcripts with complementary sequences to messenger RNA have important functions such as the stabilization and silencing of mRNA. We innestigated the regulation of natural antisense transcripts (NATs) in human HCC for their possible involvement in HCCdevelopment. We identified as up- and down-regulated in HCC tissues, compared to non-cancerous liver tissue. We also focused on HCV-associated HCCs and evaluated. Differential expression of antisense transcripts was observed between HCC and non-cancerous tissue. The sense and antisense expression profiles were analyzed with regard to histological faindings of HCC. We clarifeid different expression of NATs among the histological faindings of HCC.

肝细胞癌（HCC）发生的分子机制尚不清楚。最近的报道表明，与信使RNA互补序列的天然反义转录物具有重要的功能，如mRNA的稳定和沉默。我们研究了天然反义转录物（NATs）在人类HCC中的调控作用，以了解它们可能参与HCC的发展。与非癌性肝组织相比，我们在HCC组织中发现了上调和下调。我们还对hcv相关的hcc进行了评估。在HCC和非癌组织中观察到反义转录物的差异表达。分析肝癌组织学特征的正义和反义表达谱。我们澄清了NATs在HCC组织学特征中的不同表达。

项目成果

Elevation and characteristics of Rab30 and S100a8/S100a9 expression in an early phase of liver regeneration in the mouse

• DOI: 10.3892/ijmm.2011.614
• 发表时间: 2011-04-01
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
• 影响因子: 5.4
• 作者: Chiba, Mitsuru;Murata, Soichiro;Ohkohchi, Nobuhiro
• 通讯作者: Ohkohchi, Nobuhiro

Differential expression profiles of sense and antisense transcripts between HCV-associated hepatocellular carcinoma and corresponding non-cancerous liver tissue

• DOI: 10.3892/ijo.2012.1382
• 发表时间: 2012-06-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Nagai, Kentaro;Kohno, Keisuke;Ohkohchi, Nobuhiro
• 通讯作者: Ohkohchi, Nobuhiro

肝細胞癌におけるカスタムマイクロアレイを用いた癌関連ナチュラルアンチセンス転写産物の発現解析

使用定制微阵列对肝细胞癌中癌症相关的天然反义转录本进行表达分析

• 发表时间: 2011
• 作者: 永井健太郎;高野恵輔;千葉 満 ;朴 秀吉;村田聡一郎;安江 博;大河内信弘
• 通讯作者: 大河内信弘

Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes.

• DOI: 10.1186/1755-8794-4-42
• 发表时间: 2011-05-16
• 期刊: BMC medical genomics
• 影响因子: 2.7
• 作者: Saito R;Kohno K;Okada Y;Osada Y;Numata K;Kohama C;Watanabe K;Nakaoka H;Yamamoto N;Kanai A;Yasue H;Murata S;Abe K;Tomita M;Ohkohchi N;Kiyosawa H
• 通讯作者: Kiyosawa H

Natural antisense transcripts analysis is effective for diagnosis and carcinogenesis of hepatocellular carcinoma

天然反义转录本分析对肝细胞癌的诊断和癌变有效

• 发表时间: 2011
• 作者: Nagai K;Pak S;Kohno K;Chiba M,Murata S,Yasue H;Ohkohchi N
• 通讯作者: Ohkohchi N