Extremely small reagent handling system utilizing liposomes

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In this project we used giant unilamellar vesicles (liposomes) as a unit biochemical reactor with the volume of femtoliter for realizing minute chemical handling system. In particular we utilized the microfluidic channel and the optical tweezers to select and align a pair of liposomes in interest. Application of electric signal induced liposome fusion to mix the internal reagents, triggering the chemical reaction. Followingly the fused liposome transformed into the budded shape, separating the reaction products. Additionally lowering the ambient temperature induced the complete fission of the budded daughter vesicles. All these features materialize the chemical handling system in the femtoliter volume, which had bee unattained with the previous technologies.