GENE THERAPY FOR HEPATITIS C INFECTION--CHRONIC INFECTIOUS DISEASE MODEL

丙型肝炎感染的基因治疗--慢性传染病模型

• 批准号: 2456687
• 负责人: J W SHIH
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2456687
• 关键词: chronic disease /disorder; cytotoxic T lymphocyte; disease /disorder model; epitope mapping; gene expression; gene therapy; hematopoietic stem cells; hepatitis C; hepatitis C virus; human tissue; virus genetics

This project is part of our new initiative to develop a comprehensive research and development program to meet the challenging demands of future transfusion services. The increasing threats of emerging and re-emerging diseases, the quickened progress in the field of gene and cell therapy, and the ever-changing biotechnology have transformed the transfusion services greatly. Our viral hepatitis research and development program is built on an infectious disease model. Our specific goals for investigating gene therapy as a therapeutic approach for human hepatitis C virus (HCV) infection and developing this approach as a model for chronic infectious diseases are to (1) establish a functionally measurable gene expression in hematopoietic cells, using a viral-mediated gene delivery system for HCV gene; (2) develop lineage-specific gene expression in hematopoietic cells of HCV genes; (3) demonstrate the efficacy of lineage-specific gene expression by functional assessment with cytotoxic T lymphocyte assays and tumor killing assays directed to HCV specific epitopes; and (4) establish lineage-specific gene expression as a therapeutic modality against chronic HCV infection.

该项目是我们新举措的一部分，旨在开发 全面的研究和开发计划，以满足 未来输血服务的挑战需求。不断增加的 新出现和重新出现的疾病的威胁，加快的进展 在基因和细胞治疗领域，以及不断变化的 生物技术极大地改变了输血服务。我们的 病毒性肝炎研究和开发计划建立在 传染病模型。 我们研究基因疗法作为治疗方法的具体目标 人类丙型肝炎病毒（HCV）感染的方法和开发 这种方法作为慢性传染病的模型是（1） 建立造血功能可测量的基因表达 细胞，使用病毒介导的 HCV 基因基因传递系统； (2) 在造血细胞中发展谱系特异性基因表达 丙肝病毒基因； (3)证明谱系特异性基因的功效 通过细胞毒性 T 淋巴细胞功能评估表达 针对 HCV 特异性表位的测定和肿瘤杀伤测定； (4) 建立谱系特异性基因表达作为治疗方法 对抗慢性 HCV 感染的方法。