SALTED FISH AND NASOPHARYNGEAL CARCINOMA

这项建议是为了进行一系列的合作研究， 流行病学股和化学致癌作用股 南加州综合癌症中心和 马萨诸塞州理工学院营养与食品科学系。 本研究的总体目标是阐明 进食广式咸鱼与 鼻咽癌在中国南方的发展， 我们最近在香港进行的病例对照研究的结果表明， 我们将在广州进行类似的病例对照研究， 中华民国证实在香港的调查结果。 案件将 年时组织学确诊的50岁以下NPC病例 诊断. 对照组为年龄（5岁以内）和性别匹配的 邻里控制。 我们计划完成300个病例对照对 采访 我们将进行一项对照动物实验，其中148 近交系的Wistar-Kyoto大鼠将被喂食粉末状的咸鱼和老鼠 食物混合物，以确定广式咸鱼是否 在这个动物模型中致癌。 我们会进行实验室研究， 确定咸鱼提取物引起突变的能力， 哇巴因和8-氮鸟嘌呤耐药性以及诱导肿瘤 在哺乳动物细胞中转化。 我们会进行实验室研究， 分离和鉴定咸鱼提取物中的化合物， 模拟胃内亚硝化细菌诱变剂 条件 已确定为致突变的化合物， 将在哺乳动物细胞试验中重新测试细菌试验，包括 培养二倍体人包皮成纤维细胞，以表征其能力， 对人体细胞产生遗传毒性

This proposal is for a series of collaborative studies involving the Epidemiology Unit and the Chemical Carcinogenesis Unit of the Unviersity of Southern California Comprehensive Cancer Center and the Department of Nutrition and Food Science at the Massachusetts Institute of Technology. The overall objective of this research is to elucidate the etiologic relationship between ingestion of Cantonese-style salted fish and the development of nasopharyngeal carcinoma (NPC) in southern Chinese as suggested by the findings of our recent case-control study in Hong Kong. We will conduct a similar case-control study in Guangzhou, People's Republic of China to confirm the findings in Hong Kong. Cases will be histologically confirmed incident cases of NPC under age 50 at the time of diagnosis. Controls will be age- (within 5 years) and sex-matched neighborhood controls. We plan to complete 300 case-control pair interviews. We will conduct a controlled animal experiment in which 148 inbred Wistar-Kyoto rats will be fed a diet of powdered salted fish and rat chow mixture to determine whether Cantonese-style salted fish is carcinogenic in this animal model. We will conduct laboratory studies to determine the ability of salted fish extracts in causing mutation to ouabain and to 8-azaguanine resistance and in inducing neoplastic transformation in mammalian cells. We will conduct laboratory studies to isolate and identify the compounds in salted fish extracts which form bacterial mutagens following nitrosation under simulated gastric conditions. Compounds that have been identified as mutagenic in the facterial assays will be retested in mammalian cell assays including cultured diploid human foreskin fibroblasts to characterixe their ability to cause genotoxicity in human cells.