Dissecting a RNA-histone variant interaction and its role in splicing

剖析 RNA-组蛋白变体相互作用及其在剪接中的作用

• 批准号: DP170102525
• 负责人: Prof David Tremethick
• 金额: $ 43.59万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170102525
• 关键词: Dissecting; RNA; histone; variant; interaction

This project aims to define the molecular details of how a chromatin component, histone H2A.B, binds RNA and influences RNA splicing. This is unprecedented for histones, which are typically associated with DNA and transcriptional regulation. Over 90 per cent of human genes may be alternatively spliced. This explains how complex organisms develop from a limited set of genes, but how alternative splicing decisions are made is unclear. The intended outcome is to reveal links between chromatin, RNA splicing and gene expression regulation to explain how multicellular organisms have evolved. The translation of this knowledge will ultimately provide long-term economic and health benefits for Australia.

该项目旨在定义染色质组分，组蛋白H2A.B如何结合RNA并影响RNA剪接的分子细节。这对于通常与DNA和转录调控相关的组蛋白来说是前所未有的。超过90%的人类基因可以选择性剪接。这就解释了复杂的生物体是如何从有限的一组基因发展而来的，但选择性剪接决定是如何做出的还不清楚。预期的结果是揭示染色质，RNA剪接和基因表达调控之间的联系，以解释多细胞生物是如何进化的。这些知识的转化最终将为澳大利亚带来长期的经济和健康利益。