MOLECULAR STUDIES OF THE GENOME AND SURFACE OF SCHISTOSOMA MANSONI

曼索尼血吸虫基因组和表面的分子研究

• 批准号: 3809584
• 负责人: A SHER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809584
• 关键词: Bacillus Calmette Guerin vaccine; Brazil; Mycobacterium bovis; Salmonella; Schistosoma mansoni; T lymphocyte; animal population genetics; anthelmintics; cell bank /registry; complementary DNA; gene expression; genetic manipulation; genetic mapping; genetic markers; helminthic antigen; leukocyte activation /transformation; microorganism immunology; molecular cloning; molecular genetics; nucleic acid probes; nucleic acid sequence; parasitic disease chemotherapy; protein sequence; restriction mapping; schistosomiasis; tropomyosin; vaccines

The aim of this project is to characterize schistosome surface molecules relevant to immunity, to analyze the genome of the parasite in relation to specific biological properties of the organism and to clone important schistosome immunogens. A. Development of a paramyosin BCG recombinant cDNA's encoding paramyosin were cloned into BCG for vacome testing. B. Genetic analysis of natural schistosome populations. Using a rDNA probe, the genetic heterogeneity and geographic variation of a collection of S> mansoni isolates from Brazil was characterized. C. Creation of an expression clone bank for T epitope screening. A battery of 500 clones from a S. mansoni expression cDNA were selected for use in identifying important antigens recognized by T cells from vaccinated animals.

这个项目的目的是表征一些表面分子 与免疫力有关，分析寄生虫的基因组， 生物体的特定生物学特性，并克隆重要的 一些免疫原。 A. 卡介苗副肌球蛋白重组cDNA的构建 将其克隆入BCG中进行空泡组检测。 B。 自然群体的遗传分析。 使用rDNA 一个样本的遗传异质性和地理变异 对巴西曼氏血吸虫分离株进行了鉴定。 C. 用于T表位筛选的表达克隆库的创建。 一 一组500个克隆人选择mansoni表达cDNA， 在鉴定由接种疫苗的人的T细胞识别的重要抗原中的用途 动物