PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

这个项目的目的是研究一个广泛的物理性质 各种生物大分子，目标是将这些 大分子的结构和功能的性质。 的 重点是这些相互作用的热力学 大分子及其分子大小和形状。 分析 超浓缩和数学建模是主要的研究内容 使用的技术。 对HIV逆转录酶的研究一直致力于 研究的热力学均匀缔合的 P66亚基，以及P66与P51的异质性关联 亚单位 对色木兰素A的研究已经指向确定 这种蛋白质负责聚集的结构域， 研究了pH值和钙离子浓度对反应热力学的影响。 包含该结构域的肽的聚集特性。 CEB-63和CEB-35是亮氨酸拉链肽， 以确定表征其热力学参数， 可逆聚集 热休克蛋白和核酸的研究 一直致力于研究它们之间相互作用的本质。 关于HIV-1 RNA与核衣壳蛋白相互作用的研究， 一直致力于对这一问题进行更定量的理解 互动 对β淀粉样肽的研究已经被导向 为了了解这种肽的聚集特性及其 可能与老年痴呆症有关

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules, with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on HIV reverse transcriptase have been directed toward investigating the thermodynamics of the homogeneous association of the P66 subunit, and the heterogeneous association of P66 with the P51 subunit. Studies on chromagranin A have been directed toward determining the domain of this protein responsible for aggregation, and investigating the effects of pH and calcium ion concentration on the thermodynamics of the aggregative properties of a peptide encompassing this domain. Studies on CEB-63 and CEB-35, leucine-zipper peptides, have been directed toward determining the thermodynamic parameters characterizing their reversible aggregation. Studies on heat shock proteins and nucleic acids have been directed toward investigating the nature of their interactions. Studies on the interaction of HIV-1 RNA with nucleocapsid protein have been directed toward a more quantitative understanding of this interaction. Studies on the beta amyloid peptide have been directed toward understanding the aggregative properties of this peptide and its possible relationship to Alzheimer's disease.