IMMUNOREGULATION AND IMMUNE RECOGNITION IN FILARIASIS AND NON-FILARIAL DISEASES

这个项目的目的是描述涉及到 调节丝虫病患者的体液和细胞反应 和其他疾病状态。免疫调节研究已经检查了 微丝虫病患者抗原特异性无能现象的研究 表明这种无能是由于频率降低的 增殖细胞对寄生虫抗原的作用以及产生 抗增殖细胞因子、IL-10和转化生长因子-β。滤过性免疫斑块 已经开发出主要细胞因子的分析方法，并用于证明 在人类蠕虫感染中存在Th2、CD4+的扩张 细胞，其表型目前正在描述中。离体 寄生虫抗原驱动的抗体产生模型以及 已经使用了重组细胞因子和针对它们的中和抗体 为了了解调节体内抗体产生所需的信号 对寄生虫抗原的反应。

The purpose of this project is to delineate the mechanisms involved in regulating the humoral and cellular responses in patients with filariasis and other disease states. Immunoregulatory studies have examined the phenomenon of antigen-specific anergy in microfilaremic patients by showing this anergy to be a result of a diminished frequency of proliferating cells to parasite antigen and of the production of the antiproliferative cytokines, IL-10 and TGF-beta. Filter immunoplaque assays for the major cytokines have been developed and used to demonstrate that in helminth infections of humans there is an expansion of Th2 CD4+ cells, the phenotypes of which are currently being delineated. In vitro models of parasite-antigen driven antibody production along with recombinant cytokines and neutralizing antibodies to them have been used to understand the signals necessary to regulate antibody production in response to parasite antigen.