MOLECULAR DEFINITION OF FILARIAL ANTIGENS

丝虫抗原的分子定义

• 批准号: 3803211
• 负责人: T B NUTMAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3803211
• 关键词: Filarioidae; T lymphocyte; Wuchereria; complementary DNA; filariasis; genetic library; genetic manipulation; helminthic antigen; host organism interaction; human genetic material tag; human tissue; immediate hypersensitivity; leukocyte activation /transformation; microorganism immunology; molecular pathology; nucleic acid probes; polymerase chain reaction; serology /serodiagnosis; southern blotting

In the study of the human filariases, progress has been hampered by 1) the lack of defined parasite antigens; 2) the broad immunological cross- reactivity seen among the eight filarial species of humans; and 3) the dearth of abundant parasite material. The objectives of this project are to define and generate filarial proteins that are important in inducing parasite-specific immune responses in the human host and to understand, at a molecular level, the differences among related filarial species. cDNA and genomic libraries have been either constructed (Brugia malayi, Loa loa and Wuchereria bancrofti, Onchocerca volvulus) or made available (Onchocerca volvulus, Brugia malayi larval cDNA library) so that screening with defined patient sera, patient T cells or parasite DNA could be performed. Recombinant antigens and probes have been identified that a) encode immunoreactive and potentially protective molecule of W. bancrofti; b) can distinguish among related filarial species by restriction fragment length polymorphims, PCR or direct Southern blotting; c) identify repeated segments of either the W. bancrofti genome or that of Loa loa; d) may be of potential diagnostic importance; and e) induce immediate hypersensitivity type responses in lymphatic filarial syndromes.

在人类丝虫病的研究中，进展受到以下因素的阻碍：1） 缺乏明确的寄生虫抗原; 2）广泛的免疫交叉， 在人类的八种丝虫中观察到的反应性;以及3） 缺乏大量的寄生物。 本项目的目标是 来定义和产生丝虫蛋白， 寄生虫特异性免疫反应，并了解，在 在分子水平上，相关丝虫物种之间的差异。 已经构建了cDNA和基因组文库（马来丝虫（Brugia malayi），Loa loa和班氏吴策线虫、盘尾丝虫）或提供 （盘尾丝虫、马来丝虫幼虫cDNA文库）， 使用确定的患者血清，患者T细胞或寄生虫DNA可以被 执行。 重组抗原和探针已被鉴定为： 编码免疫反应性和潜在保护性W分子。bancrofti; B）可以通过限制性片段区分相关的丝虫物种 长度多态性，PCR或直接Southern印迹; c）鉴定重复的 无论是W。bancrofti基因组或Loa loa基因组; d）可以是 潜在的诊断重要性;和e）诱导速发型超敏反应 淋巴丝虫综合征的类型反应。