MOLECULAR DEFINITION OF FILARIAL ANTIGENS

在人类丝状病毒的研究中，进展受到了阻碍。 通过：1)缺乏明确的寄生虫抗原；2)广泛的 八种丝虫之间的免疫交叉反应 人类的物种；以及3)缺乏丰富的寄生虫材料。 该项目的目标是定义和生成丝虫 在包括寄生虫特异性免疫的过程中至关重要的蛋白质 在人类宿主中的反应，并在分子水平上理解 相关丝虫种间的差异。 已经构建了cDNA库和基因组文库(Brugia Malayi和Wuchereria bancrofti)或提供(Onchocerca 扭转)，以便用确定的患者血清或患者进行筛查 T细胞是可以进行的。重组抗原和探针 已被证实：a)在抗原中诱导T细胞反应- 特定的方式；b)对盘尾丝虫病可能有部分保护作用； C)可通过限制区分相关的丝虫物种 片段长度多态；d)编码免疫显性 W.bancrofti中的分子；以及e)识别重复的卷曲 W.bancrofti基因组的片段。

In the study of the human filariases, progress has been hampered by: 1) the lack of defined parasite antigens; 2) the broad immunological cross-reactivity seen among the eight filarial species of humans; and 3) the dearth of abundant parasite material. The objectives of this project are to define and generate filarial proteins that are important in including parasite-specific immune response in the human host and to understand, at a molecular level, the differences among related filarial species. cDNA and genomic libraries have been either constructed (Brugia malayi and Wuchereria bancrofti) or made available (Onchocerca volvulus) so that screening with defined patient sera or patient T cells could be performed. Recombinant antigens and probes have been identified that: a) induce T cell responses in an antigen- specific manner; b) may be in part protective in onchocerciasis; c) can distinguish among related filarial species by restriction fragment length polymorphisms; d) encode for an immunodominant molecular in W. bancrofti; and e) identify a tandemly repeated segment of the W. bancrofti genome.