IMMUNOREGULATION AND IMMUNE RECOGNITION IN FILARIASIS AND NON-FILARIAL DISEASES

丝虫病和非丝虫病的免疫调节和免疫识别

• 批准号: 3960474
• 负责人: T B NUTMAN
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3960474
• 关键词: B lymphocyte; Epstein Barr virus; T lymphocyte; antibody formation; antigen antibody reaction; antimicroorganism antibody; cellular immunity; clone cells; eosinophilia; filariasis; helminthic antigen; high performance liquid chromatography; human T cell lymphotropic virus type 1; human subject; human tissue; humoral immunity; immunochemistry; immunoglobulin E; immunoglobulin G; immunologic assay /test; immunoregulation; molecular cloning; onchocerciasis; parasitic disease diagnosis; radioimmunoassay; schistosomiasis; transforming virus

The purpose of this project is to delineate the mechanisms involved in regulating the humoral and cellular responses in patients with filariasis and other disease states and to determine those antigens responsible for inducing these responses. In vitro models of parasite-antigen driven antibody production as well as parasite-specific human T cell and B cell clones have been devloped to understand in more detail those mechanisms regulating antibody production (Particularly IgG and IgE) in filariasis, allergic diseases and in the normal situation. Further, HTLV-1 transformed T cell clones bearing T cell activation antigens and Fc epsilon receptors have been made in order to isolate fators involved in the T cell regulation of this isotype (IgE) which appears to be up-regulated in parasitic diseases. These T cells as well as a B cell line bearing the Fc epsilion receptor (FcER) have been used to isolate, purify, and raise antibody to the FcER which has recently enabled the gene for this receptor to be cloned molecularly. Qualitative analysis of the antigens recognized by the immune system at both a B and T cell level has been performed and T cell lines recognizing an Onchocerca volvulus species specific antigen has been constructed. Antigens recognized by human monoclonal antibodies derived from EBV-transformed patient B cells have also been identified. FPLC analysis of the antigenic components of filarial parasites has identified and helped to partially purify certain molecules which appear to regulate the immune response at both the cellular and humoral level human filariasis. Immunoblot analysis of filaria-specific IgE and IgG in loiasis, lyphatic filariasis and onchocerciasis have indicated patterns of antigen recognition which differ among groups of patients with different clinical manifestations of filariasis and among those with similar manifestations but who have been exposed to the parasite for different lengths of time.

这个项目的目的是描述涉及到 调节丝虫病患者的体液和细胞反应 和其他疾病状态，并确定那些与 诱导这些反应。 寄生虫抗原驱动抗体产生的体外模型以及 针对寄生虫的人类T细胞和B细胞克隆已经被开发出来 更详细地了解这些调节抗体产生的机制 (特别是Ig G和Ig E)在丝虫病、过敏性疾病和 正常情况下。此外，HTLV-1转化的携带T细胞的T细胞克隆 激活抗原和Fc epsilon受体已经被制造出来，以便 分离参与T细胞调节的因子(IgE) 它似乎在寄生虫病中表达上调。这些T细胞作为 以及携带Fc epsiion受体(FcER)的B细胞系 用于分离、纯化和提高新近发现的FcER抗体 使得这种受体的基因能够被分子克隆。 对免疫系统识别的抗原进行定性分析 已经进行了B和T细胞水平的检测，并识别了T细胞系 构建了螺旋体螺旋体的种特异性抗原。 人源单抗所识别的抗原 EBV转化的患者B细胞也已被鉴定。FPLC分析 丝虫的抗原性成分已经确定并帮助 部分提纯某些似乎调节免疫的分子 在细胞和体液水平对人类丝虫病的反应。 肝吸虫病患者丝虫特异性IgE和IgG的免疫印迹分析 丝虫病和盘尾丝虫病表明了抗原的类型。 对不同临床类型的患者有哪些不同的认识 丝虫病的表现以及有类似表现的人 但他们接触寄生虫的时间长短不一。