IMMUNOREGULATION AND IMMUNE RECOGNITION IN FILARIASIS AND NON-FILARIAL DISEASES

这个项目的目的是描述所涉及的机制 在调节患者的体液和细胞反应中， 丝虫病和其他疾病状态。 免疫调节研究 已经研究了抗原特异性无反应性的现象， 微丝蚴血症患者，显示这种无反应性的水平， 抗体（B细胞）和淋巴因子（T细胞）反应性。 此外，本发明还 类似的T细胞无反应性已经在活动性 盘尾丝虫病 遗传因素、抗原 组合物，以及对这种无反应性的特异性抗丝虫化疗 已经开始使用分子生物学工具和确定的抗原。 寄生虫抗原驱动的抗体产生的体外模型， 以及寄生虫特异性和HTLV-1转化的T细胞克隆， 为了更详细地了解这些机制， 调节抗体产生（特别是IgG和IgE）， 丝虫病和非丝虫病。 丝虫特异性lgE和lgG的定性分析， 淋巴丝虫病和盘尾丝虫病有指示的模式 抗原识别的差异， 丝虫病的不同临床表现。 使用这些 技术，抗原识别的患者专用 已经确定了盘尾丝虫病或盘尾丝虫病。 Fliarial物种- 人源单克隆抗体识别的特异性抗原 也已经鉴定了来自EBV转化的患者B细胞， 表征了 此外，FPLC分级分离和定性 分析丝虫的抗原成分， 确定了两组蛋白质，它们似乎调节了 免疫反应在细胞和体液水平。

The purpose of this project is to delineate the mechanisms involved in regulating the humoral and cellular responses in patients with filariaisis and other disease states. Immunoregulatory studies have examined the phenomenon of antigen-specific anergy in microfilaremic patients by showing this anergy at the level of antibody (B cell) and lymphokine (T cell) responsiveness. Further, similar T cell anergy has been found among patients with active onchocerciasis. The influence of genetic factors, antigen composition, and specific antifilarial chemotherapy on this anergy has begun using molecular biological tools and defined antigens. In vitro models of parasite-antigen driven antibody production as well as parasite-specific and HTLV-1 transformed T cell clones have been developed to understand in more detail those mechanisms regulating antibody production (particularly lgG andlgE) in filarial and non-filarial diseases. Qualitative analysis of filaria-specific lgE and lgG in loiasis, lymphatic filariasis, and onchocerciasis have indicated patterns of antigen recognition which differ among groups of patients with different clinical manifestations of filariasis. Using these techniques, antigens recognized exclusively by patients with loiasis or onchocerciasis have been identified. Fliarial species- specific antigens recognized by human monoclonal antibodies derived from EBV-transformed patient B cells have also been identified and characterized. Further, FPLC fractionation and qualitative analysis of the antigenic components of filarial parasites has identified a two sets of proteins which appears to regulate the immune response at both the cellular and humoral level.