MOLECULAR DEFINITION OF FILARIAL AND RELATED NONFILARIAL GENES AND PROTEINS

该项目的目标是定义和生成丝虫和 在诱导寄生虫特异性中起重要作用的类圆线虫蛋白 在人类宿主中的免疫反应和理解，在分子上 水平，相关蠕虫物种之间的差异。重组 已经确定了a)编码免疫反应的抗原和探针 和潜在的保护性分子；b)可以区分 在相关的丝虫物种中；c)识别W. Bancrofti、Ovolvus和Loa Loa基因组；d)具有潜在的诊断价值 重要性；以及e)负责立即 丝虫感染中的超敏型反应。此外，cDNA3 斯特氏链霉菌丝状和横纹状幼虫的文库已被 构建和表征了系统发育树，并为 这种寄生虫使用5S核糖体序列。

The objectives of this project are to define and generate filarial and strongyloides proteins that are important in inducing parasite-specific immune responses in the human host and to understand, at a molecular level, the differences among related helminth species. Recombinant antigens and probes have been identified that a) encode immunoreactive and potentially protective molecules of W. bancrofti; b) can distinguish among related filarial species; c) identify repeated segments of the W. bancrofti, O. volvulus and Loa loa genome; d) are of potential diagnostic importance; and e) are responsible for the induction of immediate hypersensitivity type responses in filarial infections. Further, cDNA libraries of filariform and rhabdiform larvae of S. stercoralis have been made and characterized and phylogenetic trees have been constructed for this parasite using 5S ribosomal sequences.