REGIONAL CEREBRAL CIRCULATION AND METABOLISM

建立了一种定量测定反应速率的方法 局部葡萄糖消耗的离散功能和结构 在有意识或麻醉的实验室动物的大脑的组成部分。 该方法是基于使用[14 C]脱氧葡萄糖作为葡萄糖的示踪剂 通过己糖激酶步骤的通量。 局部[14 C]脱氧葡萄糖-6-磷酸 CNS组织中的浓度通过定量测定来测量。 放射自显影法 由于相对的放射自显影 局部葡萄糖消耗速率可直接用于映射 在代谢上，因此在功能上，连接CNS中的结构， 该方法被用于研究能量代谢的改变， 大脑的各种功能和结构组件 生理学、药理学和病理学状态。

A method has been developed for the quantitative determination of the rates of local glucose consumption in the discrete functional and structural components of the brain in conscious or anesthetized laboratory animals. The method is based on the use of [14C]deoxyglucose as a tracer for glucose flux through the hexokinase step. Local [14C]deoxyglucose-6-phosphate concentrations in the tissues of the CNS are measured by a quantitative autoradiographic method. Inasmuch as the autoradiographs of the relative rates of local glucose consumption can be used directly for mapping metabolically, and therefore functionally, linked structures in the CNS, the method is being used to study alterations in the energy metabolism of the discrete functional and structural components of the brain in a variety of physiological, pharmacological, and pathological states.