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INTRAOCULAR CLASS II ANTIGEN EXPRESSION IN ENDOTOXIN-INDUCED UVEITIS

内毒素引起的葡萄膜炎中眼内 II 类抗原的表达

基本信息

批准号：
3898142
负责人：
A G PALESTINE
金额：
--
依托单位：
NATIONAL EYE INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

项目摘要

Endotoxin is a polysaccharide derived from the cell wall of gram negative bacteria. When injected into the footpad or the eye of a rat, it will induce an inflammatory reaction within the eye. The mechanism of this inflammation is still unclear. However, since several types of anterior uveitis in humans appear to be linked to gram negative bacteria exposure, this is considered a relative model for anterior uveitis in humans such as occurs with Reiter's syndrome. In this study, the expression of class II antigens was studied within the eyes of rats receiving Escherichia coli endotoxin by immunohistochemical techniques. We observed that the expression of class II antigens on the ciliary body and iris preceded the influx of inflammatory cells into the eye and that the inflammatory cells that entered the eye were primarily neutrophils with some monocytes. No T-cells were present in the inflammatory infiltrate. The inflammatory cellular infiltrate, could be inhibited by indomethacin or colchicine; however, this did not alter the expression of class II antigens by the iris or diary body indicating that this expression is not simply a consequence of the inflammatory infiltrate but may be intimately involved with the mechanism of the expression of endotoxin-induced uveitis. Corticosteroids were capable of suppressing both the cellular inflammatory infiltrate and the expression of class II antigens. The expression of class II antigens on nonlymphoid cells within the eye may be important in antigen presentation or may simply signal a phenotypic change on the cells due to the interaction of endotoxin with the cell membranes. The findings were compared with the expression of class II antigen in passive and active intraocular Arthus reaction. The effect of endotoxin on ocular inflammation was studied using fluorophotometry to validate the use of animal studies as a useful model. Bovine ciliary epithelium was cultured and found to express class II only in the presence of gamma interferon. Rat ciliary epithelium can function as an antigen presenting cell.

内毒素是一种来源于革兰氏阴性杆菌细胞壁的多糖，细菌当注射到老鼠的足垫或眼睛里时，引起眼睛内的炎症反应。的机理炎症仍不清楚。然而，由于几种类型的前人类的葡萄膜炎似乎与革兰氏阴性细菌接触有关，这被认为是人类前葡萄膜炎的相对模型，会出现赖特综合征在这项研究中，表达类II 在接受大肠杆菌的大鼠眼中研究抗原免疫组化法检测内毒素。我们观察到 II类抗原在睫状体和虹膜上的表达先于炎症细胞流入眼睛，主要是中性粒细胞和一些单核细胞。没有 T细胞存在于炎性浸润中。炎症吲哚美辛或秋水仙碱可抑制细胞浸润; 然而，这并没有改变虹膜II类抗原的表达，或日记体表明，这一表达不仅仅是一个后果，但也可能与炎症浸润密切相关，内毒素诱导的葡萄膜炎的表达机制。皮质类固醇能够抑制细胞炎症浸润， II类抗原的表达。 II类抗原的表达眼内非淋巴样细胞的免疫反应可能在抗原表达或可能仅仅是细胞上的表型变化的信号，内毒素与细胞膜的相互作用。这些发现是与被动和主动免疫中II类抗原的表达相比，眼内Arthus反应。内毒素对眼的影响使用荧光光度法研究炎症，以验证动物实验作为一个有用的模型。培养牛睫状体上皮并且发现仅在γ干扰素存在下表达II类。大鼠睫状体上皮可作为抗原提呈细胞。