LYMPHOCYTE MIGRATION IN EXPERIMENTAL AUTOIMMUNE UVEITIS

实验性自身免疫性葡萄膜炎中的淋巴细胞迁移

• 批准号: 3898139
• 负责人: A G PALESTINE
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3898139
• 关键词: T lymphocyte; antigens; autoimmune disorder; cell migration; disease /disorder model; helper T lymphocyte; immunization; inflammation; laboratory rat; microinjections; model design /development; peritoneal cavity; spleen transplantation; tissue /cell culture; uveitis

Experimental autoimmune uveitis (EAU) induced by immunization of rats and other experimental animals with S-antigen (a soluble antigen from the retina) is being investigated in this laboratory as a model of human intraocular inflammation. This experimental inflammation can be transferred from donor rats to naive recipients using lymphocytes harvested from the spleen or lymph nodes. Following harvest of the cells from the donors and 3 days in culture with stimulating antigen, the cells are injected into the intraperitoneal cavity and 5 to 7 days later the recipient rats develop EAU. The disease can also be transfer-red using a T-helper cell line by intraperitoneal or intraocular injection. The mechanism of transfer of disease is unclear. This work has used radioactive labeling to determine the fate of these lymphocytes after injection into the peritoneal cavity or blood during the development of uveitis. The goal of this project is to understand the initiating mechanisms of inflammation and to extend knowledge of these mechanisms to applications in human inflammations. Cells from an S-antigen-specific T-cell line migrate into the retina and cause EAU. The kinetics of this migration are being studied. S-antigen-specific cells reach the eye in greater numbers if the inflammation in the eye is induced by S-antigen than if it is induced by another mechanism.

大鼠免疫诱导的实验性自身免疫性葡萄膜炎（EAU） 其他带有S抗原（来自动物的可溶性抗原）的实验动物 视网膜）正在该实验室作为人类模型进行研究 眼内炎症。 这种实验性炎症可以 使用收获的淋巴细胞从供体大鼠转移到初始受体 来自脾脏或淋巴结。 从细胞收获后 供体和刺激抗原培养 3 天，细胞 注射到腹腔内，5~7天后 受体大鼠出现 EAU。 该疾病也可以使用以下方法转红： 通过腹膜内或眼内注射T辅助细胞系。 这 疾病转移机制尚不清楚。 本作品使用了 放射性标记以确定这些淋巴细胞的命运 在发育过程中注射到腹膜腔或血液中 葡萄膜炎。 该项目的目标是了解发起者 炎症机制并将这些机制的知识扩展到 在人类炎症中的应用。 来自 S 抗原特异性的细胞 T 细胞系迁移到视网膜并引起 EAU。 这个的动力学 正在研究移民问题。 S抗原特异性细胞到达眼睛 如果眼部炎症是由 S 抗原引起的，则数量更大 如果是由另一种机制引起的。