LYMPHOCYTE MIGRATION IN EXPERIMENTAL AUTOIMMUNE UVEITIS

实验性自身免疫性葡萄膜炎(EAU)由 S对大鼠和其他实验动物的免疫 抗原(一种来自视网膜的可溶性抗原)正在研究中 这个实验室是人类眼内炎症的模型。 这种实验性炎症可以从供者身上转移 大鼠到幼稚的接受者使用从 脾或淋巴结。在从细胞中收获细胞之后 捐献者和在刺激抗原培养三天后， 细胞被注射到腹膜腔内，五个细胞被注射到 七天后，受体大鼠发展为EAU。这种疾病可以 也可以使用T辅助细胞系通过腹膜内转移 或眼内注射。疾病传播的机制是 不清楚。这项工作使用了放射性标记的淋巴细胞 确定这些淋巴细胞在注射到 在发育过程中的腹膜腔或血液 葡萄膜炎。本项目的目标是了解 炎症机制的研究希望这些机制 可推广应用于人体炎症。单元格来自 S抗原特异性T细胞向视网膜的迁移及致病原因 真的。这种迁移的动力学正在研究中。S抗原 如果特定的细胞到达眼睛的数量更多 眼部炎症是由S抗原引起的 由另一种机制引起的。

Experimental autoimmune uveitis (EAU) is induced by immunization of rats and other experimental animals with S- antigen (a soluble antigen from the retina) is being investigated in this laboratory as a model of human intra-ocular inflammation. This experimental inflammation can be transferred from donor rats to naive recipients using lymphocytes harvested from the spleen or lymph nodes. Following harvesting of the cells from the donors and three days in culture with stimulating antigen, the cells are injected into the intra-peritoneal cavity and five to seven days later the recipient rats develop EAU. The disease can also be transferred using a T-helper cell line by intra-peritoneal or intra-ocular injection. The mechanism of transfer of disease is unclear. This work has used radioactively labeled lymphocytes to determine the fate of these lymphocytes after injection into the peritoneal cavity or blood during the process of the development of uveitis. The goal of this project is to understand the initiating mechanisms of inflammation in the hope that these mechanisms can be extended and applied to human inflammations. Cells from an S-Ag specific T cell line migrate into the retina and cause EAU. The kinetics of this migration are being studied. S-antigen specific cells reach the eye in greater numbers if the inflammation in the eye is induced by S-antigen than if it is induced by another mechanism.