LYMPHOCYTE MIGRATION IN EXPERIMENTAL AUTOIMMUNE UVEITIS

实验性自身免疫性葡萄膜炎中的淋巴细胞迁移

• 批准号: 3941662
• 负责人: A G PALESTINE
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3941662
• 关键词: T lymphocyte; antigens; autoimmune disorder; cell migration; disease /disorder model; helper T lymphocyte; immunization; inflammation; microinjections; model design /development; peritoneal cavity; radiotracer; spleen transplantation; tissue /cell culture; uveitis

Experimental autoimmune uveitis (EAU) is induced by immunization of rats and other experimental animals with S- antigen (a soluble antigen from the retina) is being investigated in this laboratory as a model of human intra-ocular inflammation. This experimental inflammation can be transferred from donor rats to naive recipients using lymphocytes harvested from the spleen or lymph nodes. Following harvesting of the cells from the donors and three days in culture with stimulating antigen, the cells are injected into the intra-peritoneal cavity and five to seven days later the recipient rats develop EAU. The disease can also be transferred using a T-helper cell line by intra-peritoneal or intra-ocular injection. The mechanism of transfer of disease is unclear. This work has used radioactively labeled lymphocytes to determine the fate of these lymphocytes after injection into the peritoneal cavity or blood during the process of the development of uveitis. The goal of this project is to understand the initiating mechanisms of inflammation in the hope that these mechanisms can be extended and applied to human inflammations. Cells from an S-Ag specific T cell line migrate into the retina and cause EAU. The kinetics of this migration are being studied. S-antigen specific cells reach the eye in greater numbers if the inflammation in the eye is induced by S-antigen than if it is induced by another mechanism.

实验性自身免疫性葡萄膜炎（EAU）由以下物质诱导： 大鼠和其他实验动物用S- 抗原（来自视网膜的可溶性抗原）正在研究中， 这个实验室作为人类眼内炎症的模型。 这种实验性炎症可以从供体转移 大鼠的幼稚受体使用淋巴细胞收获从 脾脏或淋巴结。 在从细胞中收获细胞后， 供体和三天的培养与刺激抗原， 将细胞注射到腹膜腔内， 7天后，受体大鼠发生EAU。 这种疾病可以 也可以使用T辅助细胞系通过腹膜内转移 或眼内注射。 疾病传播的机制是 不清楚 这项工作使用放射性标记的淋巴细胞， 确定这些淋巴细胞在注射到 腹膜腔或血液在发展过程中 葡萄膜炎 本项目的目标是了解 希望这些机制 可推广应用于人体炎症。 细胞 S-Ag特异性T细胞系迁移到视网膜中， EAU。 目前正在研究这种迁移的动力学。 s抗原 特定细胞到达眼睛的数量更多， 眼睛中的炎症是由S-抗原诱导的，而不是由 是由另一种机制引起的。