LYMPHOCYTE MIGRATION IN EXPERIMENTAL AUTOIMMUNE UVEITIS

实验性自身免疫性葡萄膜炎（EAU）是由免疫诱导的 大鼠和其他实验动物的S-抗原（可溶性 来自视网膜的抗原）正在本实验室进行研究 作为人类眼内炎症的模型。 该实验 炎症可以从供体大鼠转移到幼稚受体 使用从脾或淋巴结收获的淋巴细胞。 在从供体收获细胞后， 在含有刺激性抗原的培养物中，将细胞注射到 腹腔内，五到七天后， 受体大鼠发生EAU。 疾病也可以转移 使用T辅助细胞系通过腹膜内或眼内 注射 疾病传播的机制尚不清楚。 这 工作已经使用放射性标记的淋巴细胞来确定 这些淋巴细胞在注入腹膜后的命运 在葡萄膜炎的发展过程中出现空腔或血液。 这个项目的目标是了解启动机制 希望这些机制可以被扩展到 并应用于人类炎症。 来自S-Ag特异性 T细胞系迁移到视网膜并引起EAU。 动力学 这种迁移正在研究中。 S抗原特异性细胞 如果眼睛中的炎症是， 由S抗原诱导的比由另一种机制诱导的要多。

Experimental autoimmune uveitis (EAU) is induced by immunization of rats and other experimental animals with S-antigen (a soluble antigen from the retina) is being investigated in this laboratory as a model of human intra-ocular inflammation. This experimental inflammation can be transferred from donor rats to naive recipients using lymphocytes harvested from the spleen or lymph nodes. Following harvesting of the cells from the donors and three days in culture with stimulating antigen, the cells are injected into the intra-peritoneal cavity and five to seven days later the recipient rats develop EAU. The disease can also be transferred using a T-helper cell line by intra-peritoneal or intra-ocular injection. The mechanism of transfer of disease is unclear. This work has used radioactively labeled lymphocytes to determine the fate of these lymphocytes after injection into the peritoneal cavity or blood during the process of the development of uveitis. The goal of this project is to understand the initiating mechanisms of inflammation in the hope that these mechanisms can be extended and applied to human inflammations. Cells from an S-Ag specific T cell line migrate into the retina and cause EAU. The kinetics of this migration are being studied. S-antigen specific cells reach the eye in greater numbers if the inflammation in the eye is induced by S-antigen than if it is induced by another mechanism.