INTRAOCULAR CLASS II ANTIGEN EXPRESSION IN ENDOTOXIN-INDUCED UVEITIS

内毒素引起的葡萄膜炎中眼内 II 类抗原的表达

• 批准号: 3918821
• 负责人: A G PALESTINE
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3918821
• 关键词: Escherichia coli; cell membrane; corticosteroids; disease /disorder model; endotoxins; eye disorder chemotherapy; gene expression; gram negative bacteria; histochemistry /cytochemistry; histocompatibility antigens; immunochemistry; immunopharmacology; immunosuppression; indomethacin; inflammation; iris; laboratory rat; microorganism genetics; neutrophil; uvea ciliary body; uveitis

Endotoxin is a polysaccharide derived from the cell wall of gram negative bacteria. When injected into the footpad or the eye of a rat it will induce an inflammatory reaction within the eye. The mechanism of this inflammation is still unclear. However, since several types of anterior uveitis in humans appear to be linked to gram negative bacteria exposure, this is considered a relative model for anterior uveitis in humans such as Reiter's syndrome. In this study the expression of class II antigens was studied within the eyes of rats receiving E. coli endotoxin by immunohistochemical techniques. We observe that the expression of class II antigens on the ciliary body and iris preceded the influx of inflammatory cells into the eye and that the inflammatory cells that entered the eye were primarily neutrophils with some monocytes. No T-cells were present in the inflammatory infiltrate. The inflammatory cellular infiltrate could be inhibited by indomethacin or colchicine, however this did not alter the expression of class II antigens by the iris or ciliary body indicating that this expression is not simply a consequence of the inflammatory infiltrate but may be intimately involved with the mechanism of the expression of endotoxin induced uveitis. Corticosteroids were capable of suppressing both the cellular inflammatory infiltrate and the expression of class II antigens. The expression of class II antigens on nonlymphoid cells within the eye may be important in antigen presentation or may simply signal a phenotypic change on the cells due to the interaction of endotoxin with the cell membranes. The findings were compared with the expression of class II antigen in passive and active intraocular Arthus. The effect of endotoxin on ocular inflammation was studied using fluorophotometry to validate the use of animal studies as a useful

内毒素是一种来源于革兰氏阴性菌细胞壁的多糖 阴性细菌。 当注射到足垫或眼睛时， 在老鼠的眼睛内会引起炎症反应。 的 这种炎症的机制尚不清楚。 但由于 人类的几种前葡萄膜炎似乎与 革兰氏阴性菌暴露，这被认为是相对的 用于人的前葡萄膜炎例如赖特综合征的模型。 在这项研究中，研究了II类抗原的表达 在接受E.大肠杆菌内毒素 免疫组织化学技术。 我们观察到， 睫状体和虹膜上的II类抗原先于流入 炎性细胞进入眼睛， 主要是中性粒细胞， 单核细胞 在炎性浸润中不存在T细胞。 可抑制炎性细胞浸润， 吲哚美辛或秋水仙碱，但这并没有改变 虹膜或睫状体表达II类抗原 这表明这个表达式不仅仅是 炎性浸润，但可能密切参与 内毒素诱导葡萄膜炎表达的机制。 皮质类固醇能够抑制细胞内 炎症浸润和II类抗原的表达。 免疫组化显示非淋巴样细胞上的II类抗原表达， 眼睛在抗原呈递中可能是重要的， 由于以下物质的相互作用， 内毒素与细胞膜接触。 研究结果与 II类抗原在被动和主动免疫中的表达 眼内Arthus。 内毒素在眼部炎症中的作用 使用荧光光度法进行研究，以验证动物 研究作为一个有用的