REGULATION OF THE ANTIBODY RESPONSE TO MICROBIAL POLYSACCHARIDE ANTIGENS

微生物多糖抗原抗体反应的调节

• 批准号: 3960450
• 负责人: P J BAKER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3960450
• 关键词: Escherichia coli; Streptococcus pneumoniae; T lymphocyte; antibody formation; antibody specificity; autoantibody; bacterial antigens; bacterial polysaccharides; genetic regulation; hybridomas; immune tolerance /unresponsiveness; immunogenetics; immunoglobulin genes; immunologic memory; immunoregulation; immunosuppressive; leukocyte activation /transformation; lipopolysaccharides; microorganism immunology; suppressor T lymphocyte

Regulation of IgM antibody responses to several bacterial lipopolysaccharides, including Escherichia coli 0113 LPS (EC 0113-LPS), E.coli 055 LPS (EC 055-LPS) and Serratia marcesans LPS (SM-LPS) was examined. Memory to EC 0113-LPS was generated after exposure to a single subimmunogenic dose of this antigen; it developed in a cyclic manner and persisted for at least 20 days. By contrast, mice primed with a subimmunogenic dose of EC 055 LPS or SM-LPS developed profound suppression rather than memory for the antobody response to the same LPS used for priming; the development of such suppression was dose and time dependent and not due to a switch in immunoglobulin isotype. The cellular mechanisms involved in the expression of memory and suppression to LPS are being investigated in detail. The results of other studies showed that polyclonal stimulation by LPS results in the preferential activation of memory, rather than virgin B cells.

对几种细菌的IgM抗体应答的调节 脂多糖，包括大肠杆菌0113 LPS（EC 0113-LPS）， 大肠杆菌055 LPS（EC 055-LPS）和粘质沙雷氏菌LPS（SM-LPS）， 考察 对EC 0113-LPS的记忆是在暴露于单个 这种抗原的亚免疫原性剂量;它以循环方式发展， 持续了至少20天。 相比之下， 亚免疫原性剂量EC 055 LPS或SM-LPS产生显著抑制 而不是对用于相同LPS的自体反应的记忆， 引发;这种抑制的发展是剂量和时间依赖性的 而不是由于免疫球蛋白同种型的转换。 的细胞机制 参与记忆的表达和对LPS的抑制， 详细调查。 其他研究的结果表明， LPS的多克隆刺激导致优先激活 记忆，而不是处女B细胞。