CHARACTERIZATION OF ROTAVIRUSES FROM ASYMPTOMATIC HUMAN NEONATAL INFECTIONS

19株来自无症状新生儿的轮状病毒株（7株来自 英格兰、澳大利亚5人、委内瑞拉2人、瑞典5人） 在原代非洲绿色猴肾细胞中成功培养 培养物，通过空斑减少中和（PRN）试验进行血清分型， 通过间接酶联免疫吸附试验进行亚组，和 聚丙烯酰胺凝胶电泳分型。 所有19种菌株 被证明属于四种已知的人类血清型之一;血清型1 (all委内瑞拉菌株）、血清型2（所有瑞典菌株）、血清型3（所有 澳大利亚菌株）或血清型4（所有英国菌株）。 超免疫 抗委内瑞拉菌株（M37）的豚鼠抗血清中和 不仅血清型1（Wa株），而且血清型4（St.托马斯no. 3)病毒的相似程度。 英国人澳大利亚人委内瑞拉人 分离株被发现属于亚组2，瑞典菌株被 亚组1病毒。

Nineteen rotavirus strains derived from asymptomatic neonates (seven from England, five from Australia, two from Venezuela, and five from Sweden) were successfully cultivated in primary African green monkey kidney cell cultures, serotyped by plaque reduction neutralization (PRN) assay, subgrouped by indirect enzyme-linked immunosorbent assay, and electropherotyped by polyacrylamide gel electrophoresis. All 19 strains were shown to fall into one of the four known human serotypes; serotype 1 (all Venezuelan strains), serotype 2 (all Swedish strains), serotype 3 (all Australian strains), or serotype 4 (all English strains). Hyperimmune guinea pig antiserum raised against the Venezuelan strain (M37) neutralized not only serotype 1 (strain Wa) but also serotype 4 (strain St. Thomas no. 3) viruses to a similar degree. The English, Australian, and Venezuelan isolates were found to belong to subgroup 2, and the Swedish strains were subgroup 1 viruses.