ISOLATION AND SEROTYPIC CHARACTERIZATION OF HUMAN AND ANIMAL ROTAVIRUSES

在这个项目中，我们确定了各种病毒的主要抗原关系。 来自人类和动物的轮状病毒株。阐明了 轮状病毒的中和特性对于 更全面地了解轮状病毒的流行病学和 制定有效的疫苗接种战略。通过使用 各种单基因替代人×人或人×牛 轮状病毒重组体和高免豚鼠抗血清的培养 针对每一种重配子，我们成功地表征了VP4 具有代表性的人轮状病毒株(Wa， DS-1、ST3、K8和69M)，并建立了5个不同的VP4(P)血清型 基于&gt；20倍抗体差异标准，应用于 VP7血清型分类。 两只兔VP4衣壳蛋白的中和特异性 最初在美国分离的轮状病毒(C11和ALA)被发现 与人轮状病毒VP4双向抗原性相关 VP7血清6型PA169毒株(在意大利分离)和VP7血清型 8株HAL1160(芬兰分离)。

In this project we determined major antigenic relationships of various rotavirus strains derived from humans and animals. The elucidation of the neutralization specificities of rotavirus is important in order to achieve a more comprehensive understanding of rotavirus epidemiology and for formulation of an effective strategy for vaccination. By using various single gene substitution human x human or human x bovine rotavirus reassortants and hyperimmune guinea pig antiserum raised against each reassortant, we successfully characterized the VP4 neutralization specificity of representative human rotavirus strains (Wa, DS-1, ST3, K8, and 69M) and established five distinct VP4(P) serotypes based on a >20-fold antibody difference criterion that is applied to classification of VP7 serotypes. Neutralization specificities on the VP4 capsid protein of two rabbit rotaviruses (C11 and Ala) originally isolated in the U.S. were found to be related antigenically in a two-way fashion to human rotavirus VP4 of VP7 serotype 6 PA169 strain (isolated in Italy) and of the VP7 serotype 8 HAL1160 strain (isolated in Finland).