ISOLATION IN CELL CULTURE AND SEROTYPIC CHARACTERIZATION OF HUMAN ROTAVIRUSES

人类轮状病毒的细胞培养分离和血清型特征

• 批准号: 4688478
• 负责人: Y HOSHINO
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688478
• 关键词: Reoviridae disease; Rotavirus; acute infectious nonbacterial gastroenteritis; antibody neutralization test; epidemiology; genetic strain; immunogenetics; kidney cell; microorganism culture; microorganism immunology; serotyping; tissue /cell culture; viral vaccines; virus antigen; virus classification; virus genetics

This project is designed to cultivate, directly in cell cultures, a variety of human and other animal rotavirus strains from diverse geographical areas and populations in order to define serotypic diversity and similarity and to select and develop potential vaccine candidates. The newly established serotype 4 rotaviruses have been shown to play an etiological role in acute gastroenteritis, and its worldwide distribution was also documented. It was shown that there is no correlation between "family" classification based on RNA-RNA hybridization and serotype designation based on neutralization. Two human rotavirus isolates from other laboratories, which are said to represent new serotypes, as well as a bovine rotavirus isolate which is suspected of belonging to serotype 2 are under investigation in our laboratory.

这个项目的目的是培养，直接在细胞培养， 来自不同地理区域的人类和其他动物轮状病毒株 和群体，以确定血清型多样性和相似性， 选择和开发潜在的候选疫苗。 新成立的 血清型4轮状病毒已被证明在急性呼吸道感染中发挥病原学作用。 胃肠炎，其全球分布也有记录。 它 结果表明，“家庭”分类之间没有相关性 基于RNA-RNA杂交和血清型命名， 中和 从其他实验室分离的两种人轮状病毒， 据说代表了新的血清型，以及牛轮状病毒 怀疑属于血清型2的分离株， 在我们的实验室里调查。